Human Coagulation Factor V (Light Chain) Antibody

Catalog #: MAB9856 Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB9856-100
MAB9856-SP

Detection of Human Coagulation Factor V in HepG2 Human Cell Line by Flow Cytometry.

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Human Coagulation Factor V (Light Chain) Antibody Summary

Species Reactivity

Human

Specificity

Detects human Coagulation Factor V in direct ELISAs.

Source

Recombinant Monoclonal Rabbit IgG Clone # 2280A

Purification

Protein A or G purified from cell culture supernatant

Immunogen

Synthetic peptide containing human Coagulation Factor V aa1650-1700
Accession # P12259

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

ELISA

This antibody functions as an ELISA detection antibody when paired with Rabbit Anti-Human Coagulation Factor V (Light Chain) Monoclonal Antibody (Catalog # MAB98561).

This product is intended for assay development on various assay platforms requiring antibody pairs.

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Intracellular Staining by Flow Cytometry

0.25 µg/10⁶ cells

HepG2 human hepatocellular carcinoma cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry

Detection of Human Coagulation Factor V antibody in HepG2 Human Cell Line antibody by Flow Cytometry.

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Detection of Human Coagulation Factor V in HepG2 Human Cell Line by Flow Cytometry. HepG2 human hepatocellular carcinoma cell line was stained with Rabbit Anti-Human Coagulation Factor V Monoclonal Antibody (Catalog # MAB9856, filled histogram) or isotype control antibody (MAB1050, open histogram), followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (F0111). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). Staining was performed using our Staining Intracellular Molecules protocol.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Coagulation Factor V

Factor V (Coagulation Factor Five) is a 330kDa protein of the coagulation system encoded by the F5 gene. Unlike most other coagulation factors, Factor V is not enzymatically active but functions as a cofactor. Factor V is a central regulator of hemostasis. It serves as a critical cofactor for the pro-thrombinase activity of factor Xa that results in the activation of pro-thrombin to thrombin.

Long Name

Coagulation Factor V

Entrez Gene IDs

2153 (Human); 14067 (Mouse); 304929 (Rat)

Alternate Names

Activated protein C cofactor; Cf5; Cf-5; Coagulation Factor 5; coagulation factor V (proaccelerin, labile factor); coagulation factor V jinjiang A2 domain; Coagulation Factor V; F5; factor V Leiden; Factor V; FVL; PCCF; Proaccelerin, Labile Factor; RPRGL1; THPH2