Human Coagulation Factor X Antibody

Name: Human Coagulation Factor X Antibody
Brand: R&D Systems
SKU: MAB1063
Rating: 5 (1 reviews)

Catalog #: MAB1063
1 Citation 1 Review Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB1063
MAB1063-SP

All Coagulation Factor X Antibodies

Product Details

Citations (1)

FAQs

Supplemental Products

Reviews (1)

Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Coagulation Factor X Antibody Summary

Species Reactivity

Human

Specificity

Detects human Coagulation Factor X in direct ELISAs and Western blots. It recognizes both Factor X and Factor Xa.

Source

Monoclonal Mouse IgG₁ Clone # 156106

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Coagulation Factor X
Leu24-Lys488 (predicted)
Accession # P00742

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human Coagulation Factor X (Catalog # 1063-SE)
under non-reducing conditions only

Immunoprecipitation

25 µg/mL

Conditioned cell culture medium spiked with Recombinant Human Coagulation Factor X (Catalog # 1063-SE), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Coagulation Factor X

As the only known physiological activator of thrombin, Factor X is a vitamin K-dependent plasma protease that plays a pivotal role in blood coagulation. Human Factor X (rhFX) is initially synthesized in the liver as a single-chain precursor of 488 amino acids (aa) with a signal peptide and a pro region (aa 1‑40). Both the intrinsic and extrinsic pathways activate Factor X to Xa, which consists of light (aa 41‑179) and heavy (aa 235‑488) chains linked by a disulfide bond. The light chain contains a Gla and two EGF-like domains and the heavy chain corresponds to the serine protease domain.

Entrez Gene IDs

2159 (Human); 14058 (Mouse); 29243 (Rat)

Alternate Names

Cf10; Coagulation Factor X; EC 3.4.21; EC 3.4.21.6; F10; factor Xa; FX; FXa; Prothrombinase; Stuart Factor; Stuart-Prower factor

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Human Coagulation Factor X Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1