Human Coagulation Factor XI Biotinylated Antibody

Catalog #: BAF2460 Datasheet / COA / SDS

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BAF2460

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Human Coagulation Factor XI Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Coagulation Factor XI in Western blots. In Western blots, approximately 20% cross-reactivity with recombinant mouse Coagulation Factor XI is observed and less than 1% cross-reactivity with recombinant human Coagulation Factor VII and recominbnat human Coagulation Factor X is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Coagulation Factor XI
Glu19-Val625
Accession # P03951

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human Coagulation Factor XI (Catalog # 2460-SE)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Coagulation Factor XI

Coagulation factors XI and XIa refer to the pro and active forms of the same protease, respectively (1). Factor XI is synthesized in the liver and circulates in the plasma as a disulfide bond-linked dimer complexed with high molecular weight kininogen. Factor XI is converted into XIa via either the contact phase of blood coagulation or thrombin-mediated activation on the platelet surface. The resulting XIa converts factor IX into IXa, which subsequently activates factor X into Xa. Factor Xa in turn activates factor II/thrombin to complete the coagulation cascade. Patients with factor XI deficiency are prone to excessive bleeding after hemostatic challenge. There are two alternative splicing forms. Isoform 1 corresponds to the circulating plasma factor XI and isoform 2 is produced by platelets and megakaryocytes but absent from other blood cells (2). The 625 amino acid precursor of isoform 1 consists of a signal peptide (residues 1 to 18) and the mature chain (residues 19 to 625). The mature chain (XI) can be further processed into the heavy chain (residues 19 to 387) and the light chain (residues 388 to 625) (XIa). The purified rhFactor XI corresponds to isoform 1 (residues 19 to 625), which can be activated by treatment with thermolysin.

References