Human Collagen I alpha 1 Antibody

Catalog #: MAB62201 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB62201-100
MAB62201-SP

Human Collagen I alpha 1 ELISA Standard Curve.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Collagen I alpha 1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Collagen I alpha 1 in ELISAs. In direct ELISA, no cross-reactivity with human COL-2A1, COL-3A1 or COL-4A1 was observed.

Source

Monoclonal Mouse IgG₁ Clone # 816103

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Chinese Hamster Ovary cell line, CHO-derived human Collagen I alpha 1
Gln23-Lys277 + Gly1094-Leu1464
Accession # P02452

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

ELISA

This antibody functions as an ELISA capture antibody when paired with mouse anti-Human Collagen I alpha 1 monoclonal antibody (Catalog # MAB62202).This product is intended for assay development on various assay platforms requiring antibody pairs.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

ELISA

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Human Collagen I alpha 1 ELISA Standard Curve. Recombinant Human Collagen I alpha 1 protein was serially diluted 2-fold and captured by Mouse Anti-Human Collagen I alpha 1 Monoclonal Antibody (Catalog # MAB62201) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human Collagen I alpha 1 Monoclonal Antibody (Catalog # MAB62202) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Collagen I alpha 1

Type I collagen is the most abundant structural protein of connective tissues such as skin, bone and tendon. It is synthesized as a procollagen molecule which is characterized by a 300 nm triple helical domain flanked by globular N- and C-terminal propeptides (1). The triple helical domain contains Gly-Xaa-Yaa triplets where Xaa and Yaa are frequently proline and hydroxyproline, respectively. The non-helical propeptides are removed by procollagen N- and C-proteinase activities so that the mature triple helices can self-assemble into collagen fibrils that provide tensile strength to tissues (1). Type I collagen is a heterotrimer that consists of two alpha 1(I) chains and one alpha 2(I) chain, although homotrimers consisting of three identical alpha 1(I) chains have also been described (2). This recombinant mini pro-alpha 1(I) collagen consists of a shortened alpha 1(I) chain with following domain structure from N- to C-terminus: N-propeptide, N‑telopeptide, the 33 most N-terminal Gly-Xaa-Yaa repeats, the 33 most C-terminal Gly-Xaa-Yaa repeats, C-telopeptide and C-propeptide. The preparation contains a mixture of the full-length molecule, pN collagen I( alpha 1) and the C-terminal propeptide. This truncated pro-alpha 1(I) collagen is a substrate for procollagen N-proteinase and procollagen C-proteinase.

References

Canty, E.G. et al. (2005) J. Cell Sci. 118:1341.
Han, S. et al. (2008) J. Mol. Biol. 383:122.

Entrez Gene IDs

1277 (Human)

Alternate Names

Alpha-1 type I collagen; COL1A1; COL-IA1; Collagen 1 alpha 1; Collagen 1; collagen alpha 1 chain type I; collagen alpha-1(I) chain; Collagen I alpha 1; collagen of skin, tendon and bone, alpha-1 chain; Collagen Type I Alpha 1 Chain; collagen, type I, alpha 1; Collagen1; Collagen-1; EDSARTH1; OI4; pro-alpha-1 collagen type 1; Type I Procollagen Alpha 1 Chain