Human Collagen XXV alpha 1 Antibody

Catalog #: AF4317 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF4317
AF4317-SP

Detection of Human Collagen XXV alpha 1 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Collagen XXV alpha 1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Collagen XXV alpha 1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 2% cross-reactivity with recombinant human COL23A1 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Collagen XXV alpha 1
Gly189-Lys654
Accession # NP_942014

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Collagen XXV alpha 1 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and HEK293 human embryonic kidney cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Collagen XXV a1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4317) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Collagen XXV a1 at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Collagen XXV alpha 1

Collagen XXV (Collagen 25 alpha 1 chain; also CLAC-P) is a neuronal, 65‑75 kDa member of the Collagenous transmembrane (TM) subfamily of the Collagen protein family. It is synthesized as a 654 amino acid (aa) type II TM glycoprotein that contains a 33 aa cytoplasmic region (aa 1‑33) and a 600 aa extracellular domain (ECD) (aa 55‑654). The ECD contains seven Collagen-like domains. Furin cleavage between Arg112‑Glu113 generates a 70 kDa soluble fragment that homotrimerizes and binds to early A beta amyloid deposits. There are at least five multiple splice forms that impact the ECD. Amino acids 139‑146 and the seventh Collagen-like domain (aa 571‑630) are most often affected. Over aa 189‑654, human COL25A1 is 95% aa identical to mouse COL25A1.

Entrez Gene IDs

84570 (Human); 77018 (Mouse)

Alternate Names

Alzheimer disease amyloid-associated protein; AMY; CLAC; CLACP; CLAC-P; COL25A1; Collagen 25; collagen alpha-1(XXV) chain; Collagen XXV alpha 1; collagen, type XXV, alpha 1; Collagen-25; collagen-like Alzheimer amyloid plaque component; collagenous Alzheimer amyloid plaque component