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Human Common  gamma  Chain/IL-2 R gamma Antibody

Catalog #: AF284
6 Citations 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF284
AF284-SP
Cell Proliferation Induced by IL‑2 and Neutralization by Human Common gamma Chain/ IL‑2 R gamma Antibody.
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Citations (6)
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Supplemental Products
Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Common  gamma  Chain/IL-2 R gamma Antibody Summary

Species Reactivity
Human
Specificity
Detects human Common  gamma  Chain/IL‑2 R gamma in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse IL-2 R gamma is observed, less than 10% cross-reactivity with recombinant human (rh) IL-2 R beta is observed, and less than 2% cross-reactivity with rhIL-2 R alpha is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Common  gamma  Chain/IL‑2 R gamma
Leu23-Asn254
Accession # P31785
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<1.0 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human Common  gamma  Chain/IL‑2 R gamma (Catalog # 384-RG)
Flow Cytometry
0.25 µg/106 cells
Daudi human Burkitt's lymphoma cell line
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Neutralization
Measured by its ability to neutralize IL‑2-induced proliferation in the MO7e human megakaryocytic leukemic cell line. Avanzi, G. et al. (1988) Br. J. Haematol. 69:359. The Neutralization Dose (ND50) is typically 20-60 µg/mL in the presence of 30 ng/mL Recombinant Human IL‑2.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Cell Proliferation Induced by IL‑2 and Neutralization by Human Common gamma Chain/ IL‑2 R gamma Antibody. View Larger

Cell Proliferation Induced by IL‑2 and Neutralization by Human Common gamma Chain/ IL‑2 R gamma Antibody. Recombinant Human IL-2 (Catalog # 202-IL) stimulates proliferation in the MO7e human megakaryocytic leukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-2 (30 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human Common gamma Chain/ IL-2 R gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF284). The ND50 is typically 20-60 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Common gamma Chain/IL-2 R gamma

The gamma chain of the high affinity functional human IL-2 receptor complex belongs to the hematopoietin receptor family. IL-2 R gamma is a 369 amino acid residue protein consisting of a 22 residue signal sequence, a 232 residue extracellular domain, a 29 residue transmembrane domain and an 86 residue cytoplasmic domain. Although IL-2 R gamma by itself does not bind IL-2 with any appreciable affinity, it is required for IL-2 receptor signaling. Besides IL-2, the gamma  chain has been shown to be a component of the functional receptor complexes for IL-4, IL-7, IL-9 and IL-15. It has been proposed that IL-2 R gamma be designated the common gamma chain ( gamma c). The site of molecular defects in X-linked SCID (severe combined immunodeficiency) has now been mapped to the IL-2 R gamma gene.

References
  1. Minami, Y. et al. (1993) Annu. Rev. Immunol. 11:245.
  2. Noguchi, M. et al. (1993) Science 262:1877.
Long Name
IL-2 Receptor gamma
Entrez Gene IDs
3561 (Human); 16186 (Mouse)
Alternate Names
CD132 antigen; CD132; CIDX; combined immunodeficiency, X-linked; common cytokine receptor gamma chain; Common gamma Chain; common gamma-chain; cytokine receptor common subunit gamma; gamma(c); IL-2 R gamma; IL-2 receptor subunit gamma; IL2R gamma; IL-2R subunit gamma; IL2RG; IL-2RG; IMD4; interleukin 2 receptor, gamma; Interleukin-2 receptor subunit gamma; P64; SCIDX; SCIDX1; severe combined immunodeficiency

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Citations for Human Common  gamma  Chain/IL-2 R gamma Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Loss of Interleukin-13-Receptor-Alpha-1 Induces Apoptosis and Promotes EMT in Pancreatic Cancer
    Authors: Jingwei Shi, Xiao Shen, Qi Kang, Xing Yang, Maximilian Denzinger, Marko Kornmann et al.
    International Journal of Molecular Sciences
  2. Human osteoarthritic cartilage shows reduced in vivo expression of IL-4, a chondroprotective cytokine that differentially modulates IL-1beta-stimulated production of chemokines and matrix-degrading enzymes in vitro.
    Authors: Assirelli E, Pulsatelli L, Dolzani P, Platano D, Olivotto E, Filardo G, Trisolino G, Facchini A, Borzi R, Meliconi R
    PLoS ONE, 2014-05-12;9(5):e96925.
    Species: Human
    Sample Types: Whole Cells, Whole Tissue
    Applications: Flow Cytometry, IHC-Fr
  3. Interleukin-7 compartmentalizes its receptor signaling complex to initiate CD4 T lymphocyte response.
    Authors: Rose T, Pillet AH, Lavergne V, Tamarit B, Lenormand P, Rousselle JC, Namane A, Theze J
    J. Biol. Chem., 2010-02-18;285(20):14898-908.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: Flow Cytometry, Immunoprecipitation, Western Blot
  4. Identification and biochemical characterization of human plasma soluble IL-7R: lower concentrations in HIV-1-infected patients.
    Authors: Rose T, Lambotte O, Pallier C, Delfraissy JF, Colle JH
    J. Immunol., 2009-06-15;182(12):7389-97.
    Species: Human
    Sample Types: Plasma
    Applications: Immunoprecipitation
  5. Late expression of granulysin by microbicidal CD4+ T cells requires PI3K- and STAT5-dependent expression of IL-2Rbeta that is defective in HIV-infected patients.
    Authors: Zheng CF, Jones GJ, Shi M, Wiseman JC, Marr KJ, Berenger BM, Huston SM, Gill MJ, Krensky AM, Kubes P, Mody CH
    J. Immunol., 2008-06-01;180(11):7221-9.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: Flow Cytometry, Western Blot
  6. Translation of IRF-1 Restricts Hepatic Interleukin-7 Production to Types I and II Interferons: Implications for Hepatic Immunity
    Authors: Sabrina Rueschenbaum, Chengcong Cai, Matthias Schmidt, Katharina Schwarzkopf, Ulf Dittmer, Stefan Zeuzem et al.
    Frontiers in Immunology

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Human Common  gamma  Chain/IL-2 R gamma Antibody
By Anonymous on 10/26/2017
Application: WB Sample Tested: Tissue Lysates Species: Human