Human Common gamma Chain/IL-2 R gamma APC-conjugated Antibody

Name: Human Common gamma Chain/IL-2 R gamma APC-conjugated Antibody
Brand: R&D Systems
SKU: IC2841A

Catalog #: IC2841A Datasheet / COA / SDS

Discontinued Product

IC2841A has been discontinued.

View all Common gamma Chain/IL-2 R gamma products.

Detection of Common gamma Chain/IL‑2 R gamma in Jurkat Human Cell Line by Flow Cytometry.

1 Image

All Common gamma Chain/IL-2 R gamma Antibodies

Product Details

FAQs

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Common gamma Chain/IL-2 R gamma APC-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Common gamma Chain/IL‑2 R gamma in direct ELISAs.

Source

Monoclonal Mouse IgG_2A Clone # 31134

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Common gamma Chain/IL‑2 R gamma
Leu23-Asn254
Accession # P31785

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration

Sample

Intracellular Staining by Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry

Detection of Common ? Chain/IL-2 R? antibody in Jurkat Human Cell Line antibody by Flow Cytometry.

View Larger

Detection of Common gamma Chain/IL‑2 R gamma in Jurkat Human Cell Line by Flow Cytometry. Jurkat human acute T cell leukemia cell line was stained with Mouse Anti-Human Common ? Chain/IL-2 R? APC-conjugated Monoclonal Antibody (Catalog # IC2841A, filled histogram) or isotype control antibody (Catalog # IC003A, open histogram). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: Common gamma Chain/IL-2 R gamma

The gamma chain of the high affinity functional human IL-2 receptor complex belongs to the hematopoietin receptor family. IL-2 R gamma is a 369 amino acid residue protein consisting of a 22 residue signal sequence, a 232 residue extracellular domain, a 29 residue transmembrane domain and an 86 residue cytoplasmic domain. Although IL-2 R gamma by itself does not bind IL-2 with any appreciable affinity, it is required for IL-2 receptor signaling. Besides IL-2, the gamma chain has been shown to be a component of the functional receptor complexes for IL-4, IL-7, IL-9 and IL-15. It has been proposed that IL-2 R gamma be designated the common gamma chain ( gamma _c). The site of molecular defects in X-linked SCID (severe combined immunodeficiency) has now been mapped to the IL-2 R gamma gene.

References

Minami, Y. et al. (1993) Annu. Rev. Immunol. 11:245.
Noguchi, M. et al. (1993) Science 262:1877.

Long Name

IL-2 Receptor gamma

Entrez Gene IDs

3561 (Human); 16186 (Mouse)

Alternate Names

CD132 antigen; CD132; CIDX; combined immunodeficiency, X-linked; common cytokine receptor gamma chain; Common gamma Chain; common gamma-chain; cytokine receptor common subunit gamma; gamma(c); IL-2 R gamma; IL-2 receptor subunit gamma; IL2R gamma; IL-2R subunit gamma; IL2RG; IL-2RG; IMD4; interleukin 2 receptor, gamma; Interleukin-2 receptor subunit gamma; P64; SCIDX; SCIDX1; severe combined immunodeficiency