Human Complement Component C1qC Antibody

Catalog #: AF3337 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF3337
AF3337-SP

Complement Component C1qC in Human HUVEC.

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Human Complement Component C1qC Antibody Summary

Species Reactivity

Human

Specificity

Detects human Complement C1qC in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human (rh) C1qA and rhC1qB is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human C1qC
Lys115-Asp245
Accession # P02747

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human Complement Component C1qC

Immunocytochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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Complement Component C1qC in Human HUVEC. Complement Component C1qC was detected in immersion fixed HUVEC human umbilical vein endothelial cells using Goat Anti-Human Complement Component C1qC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3337) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Complement Component C1qC

C1qC is a globular secreted protein and is a subunit of complement component C1q. C1q is composed of 18 polypeptide chains of three distinct subtypes (C1qA, C1qB and C1qC) arranged as 6 disulfide-linked A-B heterodimers and 3 disulfide-linked C-C homodomers. All C1q subunits contain a C1q globular domain and one collagen-like domain. C1q is a subcomponent of complement C1 complex which comprises one C1q and the Ca²⁺-dependent serine protease tetramer C1r₂-C1s₂. C1 complex triggers the classical complement pathway. Within the C1q domain, human C1qC shares approximately 75% amino acid sequence identity with mouse and rat C1qC.

Long Name

Complement Component 1q Subcomponent Subunit C Precursor

Entrez Gene IDs

714 (Human)

Alternate Names

C1qC; C1Q-C; C1QGcomplement component 1, q subcomponent, gamma polypeptide; complement C1q subcomponent subunit C; complement component 1, q subcomponent, C chain; Complement Component C1qC; FLJ27103