Human Complement Component C2b Antibody

Name: Human Complement Component C2b Antibody
Brand: R&D Systems
SKU: MAB1936
Rating: 4.3 (4 reviews)

Catalog #: MAB1936
1 Citation 4 Reviews Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB1936
MAB1936-SP

All Complement Component C2b Antibodies

Product Details

Citations (1)

FAQs

Supplemental Products

Reviews (4)

Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Complement Component C2b Antibody Summary

Species Reactivity

Human

Specificity

Detects human Complement Component C2b in direct ELISAs and Western blots. In direct ELISAs, no cross‑reactivity with recombinant human (rh) C1r, rhC1s, rhC3r, rhC5a, rhFactor B, or rhFactor I is observed. In Western blots, this antibody recognizes rhC2 and rhC2B.

Source

Monoclonal Mouse IgG_2A Clone # 269716

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Complement Component C2
Ala21-Leu752
Accession # P06681

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human Complement Component C2b
under non-reducing conditions only

Immunoprecipitation

25 µg/mL

Conditioned cell culture medium spiked with Recombinant Human Complement Component C2b, see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Complement Component C2b

C2 is a key protease involved in the classical pathway of complement. It is synthesized as a single-chain and is proteolytically processed into two chains, C2a (aa 244‑752) and C2b (aa 21‑243). C2a consists of a vWF domain and a serine protease domain. C2b contains 3 Sushi (SCR) domains.

Entrez Gene IDs

717 (Human); 12263 (Mouse); 24231 (Rat)

Alternate Names

ARMD14; CO2; Complement Component C2b

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Human Complement Component C2b Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

rIgG1 Fc Hexamer Inhibits Antibody-Mediated Autoimmune Disease via Effects on Complement and Fc?Rs
Authors: R Spirig, IK Campbell, S Koernig, CG Chen, BJB Lewis, R Butcher, I Muir, S Taylor, J Chia, D Leong, J Simmonds, P Scotney, P Schmidt, L Fabri, A Hofmann, M Jordi, MO Spycher, S Cattepoel, J Brasseit, C Panousis, T Rowe, DR Branch, A Baz Morell, F Käsermann, AW Zuercher
J. Immunol., 2018-03-12;0(0):.
Species: Human
Sample Types: Serum
Applications: Western Blot