Human Complement Component C5/C5a Biotinylated Antibody Summary
Thr678-Arg751
Accession # P01031
Applications
Human Complement Component C5/C5a Sandwich Immunoassay
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Complement Component C5/C5a
Human complement 5a (C5a) is an enzymatically generated glycoprotein that belongs to a family of structurally and functionally related proteins known as anaphylatoxins. C5a is a 74 amino acid (aa) peptide that is created by the activity of C5a convertase on the C5 alpha -chain (1, 2). Human C5a has four alpha -helices plus three intrachain disulfide bonds that create a triple loop structure (3). In serum, proteolytic processing removes the C-terminal arginine, creating a low activity C5adesArg74 molecule (1). Human C5a is 60% and 54% aa identical to mouse and rat C5a, respectively. C5a binds to a signaling G-protein coupled receptor (C5aR/CD88) and a nonsignaling GPCR termed C5L2 (4). Activation of Cd88 results in neutrophil chemotaxis and endothelial cell activation (1, 5). It also triggers an oxidative burst in macrophages and neutrophils, and induces release of histamine in basophils and mast cells.
- Gerard, C. and N.P. Gerard (1994) Annu. Rev. Immunol. 12:775.
- DiScipio, R.G. et al. (1983) J. Biol. Chem. 258:10629.
- Huber-Lang, M.S. et al. (2003) J. Immunol. 170:6115.
- Okinaga, S. et al. (2003) Biochemistry 42:9406.
- Gerard, N.P. and C. Gerard (2002) Curr. Opin. Immunol. 14:705.
Product Datasheets
Citations for Human Complement Component C5/C5a Biotinylated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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The Influence of an Elastase-Sensitive Complement C5 Variant on Lupus Nephritis and Its Flare
Authors: CR Toy, H Song, HN Nagaraja, J Scott, J Greco, X Zhang, CY Yu, JA Tumlin, BH Rovin, LA Hebert, DJ Birmingham
Kidney international reports, 2021-06-09;6(8):2105-2113.
Species: Human
Sample Types: Plasma
Applications: ELISA Detection -
Establishing a Case for Anti-complement Therapy in Membranous Nephropathy
Authors: I Ayoub, JP Shapiro, H Song, XL Zhang, S Parikh, S Almaani, S Madhavan, SV Brodsky, A Satoskar, C Bott, L Yu, M Merchant, J Klein, JM Mejia-Vile, T Nadasdy, D Birmingham, BH Rovin
Kidney international reports, 2020-12-13;6(2):484-492.
Species: Human
Sample Types: Urine
Applications: ELISA Detection -
Generation of multiple fluid-phase C3b:plasma protein complexes during complement activation: possible implications in C3 glomerulopathies.
Authors: Ramadass, Mahalaks, Ghebrehiwet, Berhane, Smith, Richard, Kew, Richard
J Immunol, 2013-12-23;192(3):1220-30.
Species: Human
Sample Types: Serum
Applications: ELISA Development (Detection)
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