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Human Complement Component C5aR1 Fluorescein-conjugated Antibody

Catalog #: FAB3648F
1 Citation Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
FAB3648F
Detection of Complement Component C5a R1 in Human Blood Granulocytes by Flow Cytometry.
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Citations (1)
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Human Complement Component C5aR1 Fluorescein-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human Complement Component C5a R1. Stains human Complement Component C5a R1 transfected cells but not irrelevant transfectants.
Source
Monoclonal Mouse IgG2A Clone # 347214
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with human Complement Component C5a R1
Met1-Val350
Accession # P21730
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Fluorescein (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Complement Component C5a R1 antibody in Human Blood Granulocytes antibody by Flow Cytometry. View Larger

Detection of Complement Component C5a R1 in Human Blood Granulocytes by Flow Cytometry. Human peripheral blood granulocytes were stained with Mouse Anti-Human Complement Component C5a R1 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB3648F, filled histogram) or isotype control antibody (Catalog # IC003F, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Complement Component C5aR1

Complement Component C5a R1 (C5aR1), also known as C5a ligand and CD88, is a 7TM protein expressed on myeloid, endothelial, epithelial, and smooth muscle cells. C5aR binds the activated complement anaphylatoxin C5a. In established allergic environments, this triggers neutrophil and eosinophil chemotaxis and the release of proinflammatory mediators. In contrast, C5aR1/C5a interactions are protective during allergen sensitization. Human C5aR1 shares 66% amino acid sequence identity with mouse and rat C5aR.

Long Name
Complement Component C5a Receptor 1
Entrez Gene IDs
728 (Human); 12273 (Mouse)
Alternate Names
C5a anaphylatoxin chemotactic receptor; C5A; C5aR; C5a-R; C5AR1; C5ARC5a anaphylatoxin receptor; C5R1C5a ligand; CD88 antigen; CD88; complement component 5 receptor 1 (C5a ligand); complement component 5 receptor 1; complement component 5a receptor 1; Complement Component C5a R1; Complement Component C5aR1

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Citation for Human Complement Component C5aR1 Fluorescein-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. C5a receptor-dependent cell activation by physiological concentrations of desarginated c5a: insights from a novel label-free cellular assay.
    Authors: Reis E, Chen H, Sfyroera G, Monk P, Kohl J, Ricklin D, Lambris J
    J Immunol, 2012-10-05;189(10):4797-805.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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