Human Complement Factor B Biotinylated Antibody

Catalog #: BAF2739 Datasheet / COA / SDS

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BAF2739

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Complement Factor B Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Complement Factor B in Western blots. In this format, less than 2% cross-reactivity with recombinant human (rh) Factor D and rhFactor I is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Complement Factor B
Thr26-Leu764 with a Gln34Arg substitution
Accession # AAA16820

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human Complement Factor B

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Complement Factor B

Complement Factor B (also C3/C5 convertase, GBC and PBF2) is a 93-95 kDa glycoprotein member of the peptidase S1 family of enzymes. It is expressed by hepatocytes and macrophages, and serves as a substrate for complement factor D. Following binding of Factor B to membrane-bound C3b, Factor D acts on Factor B to generate a 33 kDa N-terminal fragment (Ba), plus a 60 kDa C-terminal fragment (Bb) that remains associated with C3b to createe a serine protease. Mature human Factor B is 739 amino acids (aa) in length (aa 26-764). It contains three Sushi domains (aa 35-160), one vWFA domain (aa 270-469), and a large peptidase S1 region (aa 477-757). Cleavage between Arg259Lys260 generates the Ba chain (aa #26-259) and the Bb chain (aa 260-764). There are two potential isoform variants. One shows a premature truncation after Gly589, while another shows a 79 aa substitution for aa 543-764. Over aa 26-764, human shares 85% aa identity with mouse Factor B.

Entrez Gene IDs

629 (Human); 14962 (Mouse); 294257 (Rat)

Alternate Names

BF AHUS4; BF; B-factor, properdin; BFD; C3 proaccelerator; C3 proactivator; C3/C5 convertase; CFAB; CFB; Complement Factor B; EC 3.4.21; EC 3.4.21.47; FB; FBI12; GBG; GBGCFAB; Glycine-rich beta glycoprotein; glycine-rich beta-glycoprotein; H2-Bf; PBF2; Properdin factor B