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Human Complement Factor H-related 5/CFHR5 Antibody

Catalog #: AF3845
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF3845
AF3845-SP
Product Details
Citations (2)
FAQs
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Complement Factor H-related 5/CFHR5 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Complement Factor H-related 5/CFHR5  in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Complement Factor H-related 5/CFHR5
Glu19-Glu569
Accession # Q9BXR6
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human Complement Factor H-related 5/CFHR5 (Catalog # 3845-F5)
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human Complement Factor H‑related 5/CFHR5 (Catalog # 3845-F5), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Complement Factor H-related 5/CFHR5

The human complement factor H protein family consists of the complement and immune regulators factor H, the factor H‑like protein 1 (FHL-1) and five factor H‑related proteins (FHR-1 to -5) (2). Members of this family are exclusively composed of individually folded protein domains, termed short consensus repeats (SCRs) or complement control modules. The genes of this family have been located in human chromosome 1q32, which is known as the regulator of complement activation (RCA) gene clusters (3). FHR-5 has been identified initially as a universal component of complement deposits (1), and detected in glomerular immune deposits (4). The pattern of deposits is similar to other complement components, suggesting that FHR-5 may play a role in complement activation and regulation. It is synthesized in the liver and consists of 9 SCRs. Its biological function is not understood fully. FHR-5 exhibits similar characteristics as those of factor H in heparin binding, CRP binding, and lipoprotein association (5). Weak factor I-dependent cofactor activity for C3b cleavage has also been observed (5).

References
  1. McRae, J. L. et al. (2001) J. Biol. Chem. 276:6747.
  2. Zipfel, P. F. et al. (2002) Biochem. Soc. Trans. 30:971.
  3. McRae, J. L. et al. (2002) Genetica. 114:157.
  4. Murphy, B. et al. (2002) Am. J. Kidney. Dis. 39:24.
  5. McRae, J. L. et al. (2005) J. Immunol. 174:6250.
Entrez Gene IDs
81494 (Human)
Alternate Names
CFHL5; CFHL5factor H-related protein 5; CFHR5; Complement Factor Hrelated 5; Complement Factor H-related 5; FHR5; FHR-5complement factor H-related protein 5; FHR5MGC133240; FLJ10549

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Citations for Human Complement Factor H-related 5/CFHR5 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. FHR-5 Serum Levels and CFHR5 Genetic Variations in Patients With Immune Complex-Mediated Membranoproliferative Glomerulonephritis and C3-Glomerulopathy
    Authors: Nóra Garam, Marcell Cserhalmi, Zoltán Prohászka, Ágnes Szilágyi, Nóra Veszeli, Edina Szabó et al.
    Frontiers in Immunology
  2. Factor H-related protein 5 interacts with pentraxin 3 and the extracellular matrix and modulates complement activation.
    Authors: Csincsi A, Kopp A, Zoldi M, Banlaki Z, Uzonyi B, Hebecker M, Caesar J, Pickering M, Daigo K, Hamakubo T, Lea S, Goicoechea de Jorge E, Jozsi M
    J Immunol, 2015-04-08;194(10):4963-73.
    Species: Human
    Sample Types: Serum
    Applications: Western Blot

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