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Human Complement Factor MASP3 Catalytic Domain Antibody

Catalog #: AF1724
1 Citation Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF1724
AF1724-SP
Product Details
Citations (1)
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Complement Factor MASP3 Catalytic Domain Antibody Summary

Species Reactivity
Human
Specificity
Detects human Complement Factor MASP3 Catalytic Domain in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Complement Factor MASP3
Ile450-Val721
Accession # NP_624302
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human Complement MASP3 Catalytic Domain (Catalog # 1724-SE)
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human Complement MASP3 Catalytic Domain (Catalog # 1724-SE), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Complement Factor MASP3

MASP3 is a member of the MASPs involved in mannan-binding lectin (MBL) complement pathway (1). The MBL pathway is initiated by the binding of MBL to specific carbohydrate structures found on the surface of a variety of microorganisms. Activation of the complement pathway via MBL is initiated by specific MASPs. Three MASPs have been identified and all have domain structures similar to those of C1r and C1s with a heavy chain (chain A) and a light chain (chain B). Chain A is composed of CUB1, EGF, CUB2, CCP1 and CCP2 while chain B corresponds to the catalytic domain found in many serine proteases. MASP1 and MASP3 are two alternatively spliced products of a single gene, which contain the same A chains but entirely different B chains. Distinct MASPs found in different MBL oligomers may have different biological activities. For example, MASP3, found together with MASP2, downregulates the C4 and C2 cleaving activity of MASP2. The protease activity of MASP3 is first revealed here using rhMASP3CD (2), which is inhibited by serine protease inhibitors such as Ecotin and AEBSF (Catalog # 1328-PI and EI001).

References
  1. Dahl, M.R. et al. (2001) Immunity 15:127.
  2. Cortesio, C.L. and W. Jiang (2006) Arch. Biochem. Biophys. 449:164.
Long Name
Mannan-binding lectin-Associated Serine Protease 3
Entrez Gene IDs
5648 (Human)
Alternate Names
Complement Factor MASP3; Complement factor MASP-3; Complement-activating component of Ra-reactive factor; CRARF1; CRARFMAP1; DKFZp686I01199; EC 3.4.21; EC 3.4.21.-; EC 3.4.21.104; EC 3.4.21.42; FLJ26383; mannan-binding lectin serine peptidase 1 (C4/C2 activating component ofRa-reactive factor); mannan-binding lectin serine protease 1 (C4/C2 activating component ofRa-reactive factor); mannan-binding lectin serine protease 1; Mannose-binding lectin-associated serine protease 1; Mannose-binding protein-associated serine protease; MAp44; MASP; MASP1; MASP-1; MASP3; MGC126283; MGC126284; PRSS5; Ra-reactive factor serine protease p100; RaRF; Serine protease 5

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Citation for Human Complement Factor MASP3 Catalytic Domain Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. A New Ligand-Based Method for Purifying Active Human Plasma-Derived Ficolin-3 Complexes Supports the Phenomenon of Crosstalk between Pattern-Recognition Molecules and Immunoglobulins
    Authors: Aleksandra Man-Kupisi
    PLoS ONE, 2016-05-27;11(5):e0156691.
    Species: Human
    Sample Types: Plasma
    Applications: Western Blot

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