Human Connexin 43/GJA1 Antibody

Catalog #: MAB7737 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB7737
MAB7737-SP

Detection of Connexin 43 in Human T regulatory cells by Flow Cytometry.

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Human Connexin 43/GJA1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Connexin 43/GJA1. Stains human Connexin 43/GJA1 transfectants but not irrelevant transfectants in flow cytometry.

Source

Monoclonal Mouse IgG_2A Clone # 578618

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0 transfected with human Connexin 43/GJA1
Accession # P17302

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

2.5 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of Connexin 43 in Human T regulatory cells by Flow Cytometry. Human T regulatory cells were stained with Mouse Anti-Human Connexin 43 Monoclonal Antibody (Catalog # MAB7737, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Connexin 43/GJA1

Connexin43 (Cx43; also gap junction alpha -1 protein) is a 41 - 44 kDa member of the connexin family, alpha -type subfamily, of transmembrane proteins. It is the most common type of connexin in cardiac muscle cells, and also occurs in hepatocytes, astrocytes and ovary granulosa cells. It is a 4-transmembrane protein 382 amino acids (aa) in length that contains two cytoplasmic tails. One is 12 aa in length at the N-terminus and the second is 151 aa in length at the C-terminus. Human and rat Cx43 are 98% aa identical over the entire length of the molecule. Connexins form gap junctions (GJs) which are intercellular channels between cells. Each adjacent cell contributes to a functional channel. The fundamental unit is a “connexon”, or hemi-channel, which is composed of six connexins in a sliding subunit hexamer configuration. The hexamer may be either homomeric or heteromeric. The connexon arrangement provides for the opening and closing of an intersubunit space (or pore) that allows diffusion of molecules 1 kDa or less. It is suggested that the extended C-terminus of each connexin may interact with multiple docking proteins and serve as a plug during closure. Connexons on adjacent cells interact via their extracellular loops to form a GJ channel. When open, these channels allow for the transit of small hydrophilic molecules such as ATP, glucose, IP3 and Ca++. Connexons (or hemi-channels) that do not interact with adjacent cells are also suggested to allow for small molecule transit under unusual circumstances. Cx43 activity is regulated by phosphorylation. The exact effects, however, may be context-specific. In one case, PKC phosphorylation of S368 has been found to decrease GJ channel permeability to select small molecular weight solutes. Alternatively, PKA has been suggested to be the mediator of Cx43 phosphorylation of S365, S368, S369 and S373. In this case, phosphorylation promotes channel activity.

References

Sohl, G. and K. Willecke (2004) Cardiovasc. Res. 62:228.
Willecke, K. et al. (2002) Biol. Chem. 383:725.
Giepmans, B.N.G. (2004) Cardiovasc. Res. 62:233.
Vinken, M. et al. (2006) Cell. Signal. 18:592.
Stout, C. et al. (2004) Curr. Opin. Cell Biol. 16:507.
Bao, X. et al. (2004) Am. J. Physiol. Cell. Physiol. 286:C647.
Yogo, K. et al. (2006) J. Reprod. Dev. 52:321.

Entrez Gene IDs

2697 (Human)

Alternate Names

Connexin 43; connexin-43; CX43; CX43gap junction protein, alpha-like; DFNB38; Gap junction 43 kDa heart protein; gap junction alpha-1 protein; gap junction protein, alpha 1, 43kDa (connexin 43); gap junction protein, alpha 1, 43kDa; GJA1; GJAL; HSS; ODD; ODDD; ODOD; SDTY3