Human Copeptin Antibody

Catalog #: MAB6077 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB6077
MAB6077-SP

Detection of Human Copeptin by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Copeptin Antibody Summary

Species Reactivity

Human

Specificity

Detects human Copeptin in Western blots.

Source

Monoclonal Mouse IgG₁ Clone # 579021

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Peptide corresponding to the human Copeptin precursor
Ala126-Gly140
Accession # P01185

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Copeptin by Western Blot. Western blot shows lysates of human kidney (cortex) tissue and human kidney (medulla) tissue. PVDF Membrane was probed with 2 µg/mL of Mouse Anti-Human Copeptin Monoclonal Antibody (Catalog # MAB6077) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Copeptin at approximately 7-10 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Copeptin

Copeptin is a 39 amino acid glycosylated peptide that is a proteolytic product derived from a precursor which also contains Vasopressin and Neurophysin 2 peptides. The precursor protein is synthesized and cleaved in the hypothalamus before transport to the pituitary for storage and release. Copeptin is secreted in equimolar amounts with Vasopressin. It is more stable than Vasopressin and serves as a surrogate indicator of Vasopressin release. Vasopressin plays a major role in blood pressure regulation through control of water retention in the kidney and vascular tone. Serum levels of Copeptin are associated with metabolic syndrome, insulin resistance, sepsis, and heart dysfunction following myocardial infarction. Human Copeptin shares 79% sequence identity with mouse and rat Copeptin.

Entrez Gene IDs

551 (Human)

Alternate Names

ADH;ARVP;AVP;AVP-NPII;AVRP;Vasopressin-neurophysin 2-copeptin;VP; Copeptin