Human CPT1A Antibody

Catalog #: MAB11570 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB11570-100
MAB11570-SP

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Human CPT1A Antibody Summary

Species Reactivity

Human

Specificity

Detects a synthetic peptide corresponding to residues surrounding amino acid 501 of human CPT1A protein in direct ELISA.

Source

Monoclonal Mouse IgG2b Clone # 1081618

Immunogen

CPT1A containing synthetic peptide
Accession # P50416

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Applications

Recommended Concentration

Sample

Immunohistochemistry

3-25 µg/mL

Immersion fixed paraffin-embedded sections of human kidney

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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Detection of CPT1A in Human Kidney. CPT1A was detected in immersion fixed paraffin-embedded sections of human kidney using Mouse Anti-Human CPT1A Monoclonal Antibody (Catalog # MAB11570) at 5 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001) or the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute lyophilized material at 0.2 mg/ml in sterile PBS. For liquid material, refer to CoA for concentration.

Stability & Storage

Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: CPT1A

CPT1A is one of the isoforms of CPT1. CPT1 is a mitochondrial enzyme responsible for the formation of acyl carnitines. CPT1A is the rate-limiting enzyme in the fatty acid beta -oxidation, allowing fatty acids to enter the mitochondrial matrix for oxidation. Deficiency or abnormal regulation in this process can result in diseases like metabolic disorders and cancer including but not limited to: CPT1A deficiency, metabolic syndrome, obesity, type 2 diabetes, vascular disease, heart failure, non‑alcoholic fatty liver disease, MS, and renal fibrosis. CPT1A is expressed on the outer mitochondrial membrane of most tissues but predominates in lipogenic tissues such as liver. Genetic, physiological, and dietary modulators are all involved in the regulation of CPT1A.

References

Brown NF, Hill JK, Esser V, Kirkland JL, Corkey BE, Foster DW, McGarry JD. Mouse white adipocytes and 3T3-L1 cells display an anomalous pattern of carnitine palmitoyltransferase (CPT) I isoform expression during differentiation. Inter-tissue and inter-species expression of CPT I and CPT II enzymes. Biochem J. 1997 Oct 1;327 ( Pt 1)(Pt 1):225-31. doi: 10.1042/bj3270225. PMID: 9355756; PMCID: PMC1218784.
Liang K. Mitochondrial CPT1A: Insights into structure, function, and basis for drug development. Front Pharmacol. 2023 Mar 23;14:1160440. doi: 10.3389/fphar.2023.1160440. PMID: 37033619; PMCID: PMC10076611.

Long Name

Carnitine O-palmitoyltransferase 1, liver isoform

Entrez Gene IDs

1374 (Human); 12894 (Mouse); 25757 (Rat)

Alternate Names

carnitine O-palmitoyltransferase 1, liver isoform; Carnitine O-palmitoyltransferase I, liver isoform; carnitine palmitoyltransferase 1A (liver); Carnitine palmitoyltransferase 1A; carnitine palmitoyltransferase I, liver; CPT I; CPT1; CPT1A; CPT1-L; CPT1-LEC 2.3.1.21; CPTI-L; EC 2.3.1; EC 2.3.1.21; L-CPT1