Human CREB Antibody

Name: Human CREB Antibody
Brand: R&D Systems
SKU: MAB2989
Rating: 4 (1 reviews)

Catalog #: MAB2989 1 Review Datasheet / COA / SDS

Discontinued Product

MAB2989 has been discontinued.

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All CREB Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CREB Antibody Summary

Species Reactivity

Human

Specificity

Detects human CREB in direct ELISAs.

Source

Monoclonal Mouse IgG₁ Clone # 321120

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human CREB
Lys136-Asp341
Accession # P16220

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Immunohistochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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CREB in Human Prostate. CREB was detected in immersion fixed paraffin-embedded sections of human spinal prostate array using Human CREB Monoclonal Antibody (Catalog # MAB2989) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CREB

The cAMP response element binding protein (CREB), also known as CREB1, belongs to the bZIP family of transcription factors. Each bZIP family member contains a basic domain that mediates DNA binding and a leucine zipper domain that facilitates dimerization. Within the promoter of target genes, CREB dimers bind cAMP response elements, defined by the palindromic consensus sequence TGACGTCA.

Long Name

cAMP Response Element-binding Protein

Entrez Gene IDs

1385 (Human); 12912 (Mouse)

Alternate Names

active transcription factor CREB; cAMP responsive element binding protein 1; cAMP-response element-binding protein-1; cAMP-responsive element-binding protein 1; CREB; CREB1; CREB-1; cyclic AMP-responsive element-binding protein 1; MGC9284; transactivator protein