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Human CRISP-2 Antibody

Catalog #: MAB2575
1 Citation Datasheet / COA / SDS
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MAB2575
MAB2575-SP
Product Details
Citations (1)
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CRISP-2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human CRISP-2 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human CRISP-3 is observed.
Source
Monoclonal Mouse IgG2B Clone # 350814
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human CRISP-2
Lys22-Tyr243
Accession # P16562
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human CRISP‑2

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CRISP-2

Human CRISP-2, the ortholog of rodent testis specific protein 1 (Tpx1) is a secreted protein expressed in testis. It is a cell adhesion molecule that mediates interactions between spermatogenic cells and Sertoli cells. Mature human CRISP-2 shares 86%, 73% and 71% amino acid sequence identity with the equine, mouse and rat proteins, respectively. Human CRISP-2 also shares 71% amino acid sequence homology with human CRISP-3.

Long Name
Cysteine-rich Secretory Protein 2
Entrez Gene IDs
7180 (Human); 22024 (Mouse)
Alternate Names
Cancer/testis antigen 36; CRISP2; CRISP-2; CRISP-2GAPDL5; CT36TSP1; cysteine-rich secretory protein 2; GAPDL5; glyceraldehyde-3-phosphate dehydrogenase-like 5; testis specific protein 1 (probe H4-1 p3-1); Testis-specific protein TPX-1; TPX1; TPX1MGC111136; TSP1

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Citation for Human CRISP-2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Purification and characterization of CRISP-3 from human seminal plasma and its real-time binding kinetics with PSP94
    Authors: Smita D Mahale
    J. Chromatogr. B Analyt. Technol. Biomed. Life Sci., 2016-10-26;1039(0):59-65.
    Species: Human
    Sample Types: Protein
    Applications: Western Blot

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