Home / CRTAM / Human CRTAM Antibody

Human CRTAM Antibody

Catalog #: MAB16951
4 Citations Datasheet / COA / SDS
Clone 210213 was used by HLDA to establish CD designation
Catalog # Availability Size / Price Qty
MAB16951
MAB16951-SP
Product Details
Citations (4)
FAQs
Supplemental Products
Reviews
Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CRTAM Antibody Summary

Species Reactivity
Human
Specificity
Detects human CRTAM in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse CRTAM is observed.
Source
Monoclonal Mouse IgG2B Clone # 210213
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human CRTAM
Ser18-Ser286
Accession # O95727
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human CRTAM Fc Chimera (Catalog # 1695-CR)
Flow Cytometry
2.5 µg/106 cells
Human CD8+ T cells treated with PMA, Ca2+ and ionomycin
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Loading...
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CRTAM

CRTAM (Class I-restricted T cell-associated molecule) is a nectin family member of the immunoglobulin superfamily that is expressed by activated CD8+ and NK T cells (1‑4). NK activation receptor engagement, but not cytokines, also induce NK CRTAM expression (4, 5). CRTAM is found in spleen, thymus, small intestine, peripheral blood, and surprisingly, in brain where it is highly expressed by Purkinje cells of the cerebellum (1, 2). Human CRTAM is a 393 amino acid (aa), 80 kDa type I transmembrane glycoprotein with a 17 aa signal sequence, a 269 aa extracellular domain (ECD), a 21 aa transmembrane segment and an 84 aa cytoplasmic domain. The ECD has one V-type and one C1-type Ig-like domain, while the cytoplasmic region shows a potential class I PDZ domain (1‑5). Human CRTAM ECD shows 70%, 43% and 63% aa identity with mouse, rat and canine CRTAM ECD, respectively, but 73‑78% aa identity within the Ig-like domains. The V-type Ig-like domain mediates interaction with the corresponding domain on another nectin family member, IGSF4 (also called TSLC-1, Necl-2, Syncam or SgIGSF) (4, 5). CRTAM is a homodimer on the cell surface but does not show homotypic binding in trans (3‑5). The high affinity of CRTAM/IGSF4 adhesion allows CRTAM to disrupt IGSF4 homotypic interactions (3‑5). IGSF4 and T cell receptor co-engagement of CRTAM-expressing CD8+ cells induces increased IFN-gamma or IL-22 production (3, 4). A role in cancer surveillance through NK cell-mediated rejection of IGSF4-expressing tumors has been proposed (3‑5). IGSF4 is expressed broadly, including on epithelia, neurons, a subset of tonsillar B cells (4, 5), and a rare splenic T zone-restricted BCDA3+ dendritic cell population which interacts with CRTAM (3).

References
  1. Kennedy, J. et al. (2000) J. Leukoc. Biol. 67:725.
  2. Patino-Lopez, G. et al. (2006) J. Neuroimmunol. 171:145.
  3. Galibert, L. et al. (2005) J. Biol. Chem. 280:21955.
  4. Boles, K. S. et al. (2005) Blood 106:779.
  5. Arase, N. et al. (2005) Int. Immunol. 17:1227.
Long Name
Class I-restricted T-cell-associated Molecule, Nectin
Entrez Gene IDs
56253 (Human); 54698 (Mouse); 102125896 (Cynomolgus Monkey)
Alternate Names
CD355 antigen; CD355; class I MHC restricted T cell associated molecule; class-I MHC-restricted T cell associated molecule; Class-I MHC-restricted T-cell-associated molecule; CRTAM; cytotoxic and regulatory T cell molecule; cytotoxic and regulatory T-cell molecule

Product Datasheets

You must select a language.

x
Product Datasheet
COA
SDS

Citations for Human CRTAM Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
Filter your results:

Filter by:

  1. CADM1 is a key receptor mediating human mast cell adhesion to human lung fibroblasts and airway smooth muscle cells.
    Authors: Moiseeva, Elena P, Roach, Katy M, Leyland, Mark L, Bradding, Peter
    PLoS ONE, 2013-04-19;8(4):e61579.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. CRTAM receptor engagement by Necl-2 on tumor cells triggers cell death of activated Vgamma9Vdelta2 T cells.
    Authors: Dessarthe B, Thedrez A, Latouche J, Cabillic F, Drouet A, Daniel P, de La Pintiere C, Catros V, Toutirais O
    J Immunol, 2013-03-25;190(9):4868-76.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. New B-cell CD molecules.
    Authors: Matesanz-Isabel J, Sintes J, Llinas L, de Salort J, Lazaro A, Engel P
    Immunol. Lett., 2010-10-07;134(2):104-12.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Analysis of HLDA9 mAbs on plasmacytoid dendritic cells.
    Authors: Cabezon R, Sintes J, Llinas L, Benitez-Ribas D
    Immunol. Lett., 2010-10-07;134(2):167-73.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for Human CRTAM Antibody

There are currently no reviews for this product. Be the first to review Human CRTAM Antibody and earn rewards!

Have you used Human CRTAM Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review