Human CtBP1 Antibody

Catalog #: AF4639 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF4639
AF4639-SP

Detection of Human CtBP1 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CtBP1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human and mouse CtBP1 in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human CtBP2 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human CtBP1
Met1-Leu440
Accession # Q13363

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human CtBP1 by Western Blot. Western blot shows lysates of A431 human epithelial carcinoma cell line, A549 human lung carcinoma cell line, HeLa human cervical epithelial carcinoma cell line, and PT18 mouse mast/basophil cell line. PVDF membrane was probed with 1 µg/mL Goat Anti-Human CtBP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4639) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). For additional reference, recombinant human CtBP1 and CtBP2 (5 ng/lane) were included. A specific band for CtBP1 was detected at approximately 48 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CtBP1

CtBP1 (C terminal binding protein 1) was originally identified through its binding to the Adenovirus E1A oncoprotein via a 5 amino acid motif, PLDLS, which repressed E1A induced oncogenesis and cellular transformation. In the nucleus, CtBP1 functions predominantly as a transcriptional corepressor in conjunction with a variety of DNA binding repressors.Interaction with Pnn/DRS may modulate repression of E-cadherin by CtBP1. Recruitment of CtBP1 and HDAC to Smad1/Smad4 complexes by Smad6 results in inhibition of BMP stimulated gene expression. The interaction of CtBP1 with polycomb group proteins suggests it is a component of the chromatin remodeling machinery and transcriptional repression. Cytosolic CtBP1 has also been shown to be involved with CNS synapses, membrane trafficking, and regulation of the microtubule cytoskeleton.

Long Name

C-terminal-Binding Protein 1

Entrez Gene IDs

1487 (Human); 13016 (Mouse); 29382 (Rat)

Alternate Names

BARS; brefeldin A-ribosylated substrate; CTBP; CtBP1; C-terminal binding protein 1; C-terminal-binding protein 1; EC 1.1.1; EC 1.1.1.-; EC 1.1.1.158; MGC104684