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Human CTRP5/C1qTNF5 Antibody

Catalog #: AF3167
1 Citation Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF3167
AF3167-SP
Detection of CTRP5/C1qTNF5 in A172 Human Cell Line by Flow Cytometry.
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Human CTRP5/C1qTNF5 Antibody Summary

Species Reactivity
Human
Specificity
Detects human CTRP5/C1qTNF5 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human CTRP4 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human CTRP5/C1qTNF5
Ser16-Ala243
Accession # AAH29485
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human CTRP5/C1qTNF5
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Intracellular Staining by Flow Cytometry
0.25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry Detection of CTRP5/C1qTNF5 antibody in A172 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of CTRP5/C1qTNF5 in A172 Human Cell Line by Flow Cytometry. A172 human glioblastoma cell line was stained with Goat Anti-Human CTRP5/C1qTNF5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3167, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CTRP5/C1qTNF5

C1qTNF5, also known as CTPR5, is a secreted protein that belongs to the highly conserved family of Acrp30/Adiponectin paralogs known as C1q and TNF-related protein family. All family members share a modular organization comprising an N-terminal signal peptide, a short variable region, a collagenous domain and a
C‑terminal globular domain. C1qTNF proteins are predicted to have trimeric structures that assemble into hexameric and higher order molecular forms. Human and mouse C1qTNF5 share 94% amino acid sequence identity.

Long Name
C1q And Tumor Necrosis Factor Related Protein 5
Entrez Gene IDs
114902 (Human); 235312 (Mouse)
Alternate Names
C1q and tumor necrosis factor related protein 5; C1qTNF5; Complement C1q Tumor Necrosis Factor-Related Protein 5 Precursor Variant 3; CTRP5; CTRP5LORDDKFZp586B0621; LORD 6; Myonectin 3

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Citation for Human CTRP5/C1qTNF5 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Molecular, biochemical and functional characterizations of C1q/TNF family members: adipose-tissue-selective expression patterns, regulation by PPAR-gamma agonist, cysteine-mediated oligomerizations, combinatorial associations and metabolic functions.
    Authors: Wong GW, Krawczyk SA, Kitidis-Mitrokostas C, Revett T, Gimeno R, Lodish HF
    Biochem. J., 2008-12-01;416(2):161-77.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Western Blot

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