Human CTRP9/C1qTNF9 Antibody

Catalog #: AF6537 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF6537-100
AF6537-SP

Detection of Human CTRP9/C1qTNF9 by Western Blot.

1 Image

Product Details

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CTRP9/C1qTNF9 Antibody Summary

Species Reactivity

Human

Specificity

Detects human CTRP9/C1qTNF9 in direct ELISAs.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human CTRP9/C1qTNF9
Asn16-Pro333
Accession # P0C862

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

View Larger

Detection of Human CTRP9/C1qTNF9 by Western Blot. Western blot shows human serum. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Human CTRP9/C1qTNF9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6537) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CTRP9/C1qTNF9 at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CTRP9/C1qTNF9

C1qTNF9, also known as CTRP9, is one of several Adiponectin/Acrp30 paralogs which comprise the C1q and TNF-related protein family. All family members share a modular organization comprising a short variable region, a collagenous domain, and a C1q-like globular domain. C1qTNF9 is a 40 kDa glycoprotein that contains multiple hydroxylated proline residues in its collagenous region. It circulates as a homotrimer and higher order multimers as well as in heterotrimers with Adiponectin. It is preferentially expressed in adipose tissue and plays a role in glucose homeostasis. Human C1qTNF9 shares 85% amino acid sequence identity with mouse and rat C1qTNF9.

Long Name

C1q and Tumor Necrosis Factor Related Protein 9

Entrez Gene IDs

338872 (Human); 239126 (Mouse)

Alternate Names

AQL1; C1q and tumor necrosis factor related protein 9; C1qTNF9; C1QTNF9AComplement C1q tumor necrosis factor-related protein 9; complement C1q tumor necrosis factor-related protein 9A; CTRP9; MGC48915