Human CTRP9/C1qTNF9 Antibody

Catalog #: AF6537 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF6537-100
AF6537-SP

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Detection of Human CTRP9/C1qTNF9 by Western Blot.

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Human CTRP9/C1qTNF9 Antibody Summary

Species Reactivity

Human

Specificity

Detects human CTRP9/C1qTNF9 in direct ELISAs.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human CTRP9/C1qTNF9
Asn16-Pro333
Accession # P0C862

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human CTRP9/C1qTNF9 by Western Blot. Western blot shows human serum. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Human CTRP9/C1qTNF9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6537) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CTRP9/C1qTNF9 at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CTRP9/C1qTNF9

C1qTNF9, also known as CTRP9, is one of several Adiponectin/Acrp30 paralogs which comprise the C1q and TNF-related protein family. All family members share a modular organization comprising a short variable region, a collagenous domain, and a C1q-like globular domain. C1qTNF9 is a 40 kDa glycoprotein that contains multiple hydroxylated proline residues in its collagenous region. It circulates as a homotrimer and higher order multimers as well as in heterotrimers with Adiponectin. It is preferentially expressed in adipose tissue and plays a role in glucose homeostasis. Human C1qTNF9 shares 85% amino acid sequence identity with mouse and rat C1qTNF9.

Long Name

C1q and Tumor Necrosis Factor Related Protein 9

Entrez Gene IDs

338872 (Human); 239126 (Mouse)

Alternate Names

AQL1; C1q and tumor necrosis factor related protein 9; C1qTNF9; C1QTNF9AComplement C1q tumor necrosis factor-related protein 9; complement C1q tumor necrosis factor-related protein 9A; CTRP9; MGC48915

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Product Datasheet

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