Human Cubilin Biotinylated Antibody

Catalog #: BAF3700 Datasheet / COA / SDS

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BAF3700

All Cubilin Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Cubilin Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Cubilin in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human (rh) Notch-2 and rhNotch-3 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human Cubilin
Ser36-Gly126
Accession # O60494

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human Cubilin

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cubilin

Cubilin is a 460 kDa protein that is a component of a multiligand endocytic receptor complex, which also contains amnionless, megalin and LRP2. The receptor complex is expressed in several absorptive epithelia, including kidney proximal tubule, yolk sac, and the small intestine. Human Cubilin is synthesized as a 3623 residue precursor with a signal peptide, an amino-terminal domain, eight EGF-like repeats and 27 Cub domains. Co-expression with amnionless is required for the membrane anchoring, ER export and internalizing action of Cubilin. Within the region used as the immunogen, human, mouse and rat Cubilin share approximately 76% amino acid sequence homology.

Entrez Gene IDs

8029 (Human); 65969 (Mouse); 80848 (Rat)

Alternate Names

cubilin; cubilin (intrinsic factor-cobalamin receptor); Cubilin; CUBN; Cubulin; gp280; IFCR; MGA1