Human CX3CL1/Fractalkine Chemokine Domain Biotinylated Antibody

Catalog #: BAM365
5 Citations Datasheet / COA / SDS

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BAM365	In Stock	100 ug / $529

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Citations (5)

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CX3CL1/Fractalkine Chemokine Domain Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Detects human CX3CL1 in ELISAs and Western blots. In ELISAs, this antibody shows approximately 8% cross-reactivity with recombinant mouse (rm) CXCL6 and less than 0.2% cross-reactivity with rhCXCL6, rhCXCL9, and rrCX3CL1.

Source

Monoclonal Mouse IgG₁ Clone # 51637

Purification

Protein A or G purified from ascites

Immunogen

NS0-derived and E.coli-derived Recombinant Human Fractalkine
Gln25-Arg339
Accession # P78423

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human CX3CL1/Fractalkine Full Length (Catalog # 365-FR)

Human CX3CL1/Fractalkine Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Detection (Matched Antibody Pair)

0.5-2.0 µg/mL

Use in combination with:

Capture Reagent: Human CX3CL1/Fractalkine Chemokine Domain Antibody (Catalog # MAB3652)

Standard: Recombinant Human CX3CL1/Fractalkine (Full Length) Protein (Catalog # 365-FR)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CX3CL1/Fractalkine

Fractalkine is a type I membrane protein in which a chemokine domain possessing a unique C-X3-C cysteine motif is tethered on a long mucin-like stalk. It can also be released as a soluble molecule upon proteolysis at a membrane proximal site.

Entrez Gene IDs

6376 (Human); 20312 (Mouse); 89808 (Rat); 102117496 (Cynomolgus Monkey)

Alternate Names

ABCD-3; C3Xkine; chemokine (C-X3-C motif) ligand 1; CX3C membrane-anchored chemokine; CX3CL1; CXC3; CXC3C; FKN; Fractalkine; Neurotactin; neurotactin); NTNsmall inducible cytokine subfamily D (Cys-X3-Cys), member 1 (fractalkine; NTTSmall-inducible cytokine D1; SCYD1C-X3-C motif chemokine 1; small inducible cytokine subfamily D (Cys-X3-Cys), member-1

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human CX3CL1/Fractalkine Chemokine Domain Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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Shear Stress Counteracts Endothelial CX3CL1 Induction and Monocytic Cell Adhesion
Authors: A Babendreye, L Molls, D Dreymuelle, S Uhlig, A Ludwig
Mediators Inflamm., 2017-03-26;2017(0):1515389.
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Development (Detection)
Multiplex serum protein analysis reveals potential mechanisms and markers of response to hyperimmune caprine serum in systemic sclerosis
Authors: N Quillinan, KE Clark, B Youl, J Vernes, D McIntosh, S Haq, CP Denton
Arthritis Res. Ther, 2017-03-07;19(1):45.
Species: Human
Sample Types: Serum
Applications: ELISA Development (Detection)
Thrombin-induced expression of endothelial CX3CL1 potentiates monocyte CCL2 production and transendothelial migration.
Authors: Popovic M, Laumonnier Y, Burysek L, Syrovets T, Simmet T
J. Leukoc. Biol., 2008-04-24;84(1):215-23.
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Development
Regulated shedding of transmembrane chemokines by the disintegrin and metalloproteinase 10 facilitates detachment of adherent leukocytes.
Authors: Hundhausen C, Schulte A, Schulz B, Andrzejewski MG, Schwarz N, von Hundelshausen P, Winter U, Paliga K, Reiss K, Saftig P, Weber C, Ludwig A
J. Immunol., 2007-06-15;178(12):8064-72.
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Development
Fractalkine mediates natural killer-dependent antitumor responses in vivo.
Authors: Lavergne E, Combadiere B, Bonduelle O, Iga M, Gao JL, Maho M, Boissonnas A, Murphy PM, Debre P, Combadiere C
Cancer Res., 2003-11-01;63(21):7468-74.
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Development