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Human CXCL10/IP-10/CRG-2 PE-conjugated Antibody

Catalog #: IC266P
4 Citations Datasheet / COA / SDS
Newer Version Available: MAB266
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Discontinued Product

IC266P has been discontinued and is replaced by MAB266.

Detection of CXCL10/IP‑10/CRG‑2 in Human Blood Monocytes by Flow Cytometry.
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Citations (4)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CXCL10/IP-10/CRG-2 PE-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human CXCL10/IP-10/CRG-2 in ELISAs and Western blots. In Western blots, does not cross-react with recombinant human CXCL1, 2, 3, 5, 8, 9, 12/SDF-1 beta, recombinant mouse CXCL1, or 2.
Source
Monoclonal Mouse IgG1 Clone # 33036
Purification
Protein A or G purified from ascites
Immunogen
E. coli-derived recombinant human CXCL10/IP-10/CRG-2
Val22-Pro98
Accession # P02778.2
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry Detection of CXCL10/IP‑10/CRG‑2 antibody in Human Blood Monocytes antibody by Flow Cytometry. View Larger

Detection of CXCL10/IP‑10/CRG‑2 in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes treated with LPS were stained with Mouse Anti-Human CXCL10/IP-10/CRG-2 PE-conjugated Monoclonal Antibody (Catalog # IC266P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CXCL10/IP-10/CRG-2

CXCL10 was originally identified as an IFN-gamma -inducible gene in monocytes, fibroblasts and endothelial cells. It has since been shown that CXCL10 mRNA is also induced by LPS, IL-1 beta, TNF-alpha, IL-12, and viruses. Additional cell types that have been shown to express CXCL10 include activated T-lymphocytes, splenocytes, keratinocytes, osteoblasts, astrocytes, and smooth muscle cells. CXCL10 is also expressed in psoriatic and lepromatous lesions of skin. The mouse homologue of human CXCL10, CRG-2, has been cloned and shown to share approximately 67% amino acid sequence identity with human CXCL10. Human CXCL10 cDNA encodes a 98 amino acid (aa) residue precursor protein with a 21 aa residue signal peptide that is cleaved to form the 77 aa residue secreted protein. The amino acid sequence of CXCL10 identified the protein as a member of the chemokine alpha subfamily that lacks the ELR domain. CXCL10 has been shown to be a chemoattractant for activated T-lymphocytes. CXCL10 has been reported to be a potent inhibitor of angiogenesis and to display a potent thymus-dependent antitumor effect. A chemokine receptor specific for CXCL10 and Mig has been cloned and shown to be highly expressed in IL-2-activated T-lymphocytes.

References
  1. Loetscher, M. et al. (1996) J. Exp. Med. 184:963.
  2. Wang, X. et al. (1996) J. Biol. Chem. 271:24286.
Entrez Gene IDs
3627 (Human); 15945 (Mouse)
Alternate Names
C7; chemokine (C-X-C motif) ligand 10; CRG2; CRG-2; CXCL10; gIP-10; IFI10; INP10; IP-10; mob-1; SCYB10

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Citations for Human CXCL10/IP-10/CRG-2 PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. High-content cytometry and transcriptomic biomarker profiling of human B-cell activation.
    Authors: Hennig C, Ilginus C, Boztug K, Skokowa J, Marodi L, Szaflarska A, Sass M, Pignata C, Kilic S, Caragol I, Baumann U, Klein C, Welte K, Hansen G
    J Allergy Clin Immunol, 2013-09-05;133(1):172-80.e1-10.
    Species: Human
    Sample Types: Whole Cells
    Applications: Chip Cytometry
  2. Functional OCT4-specific CD4 and CD8 T cells in healthy controls and ovarian cancer patients.
    Authors: Di J, Massuger L, Duiveman-de Boer T, Zusterzeel P, Figdor C, Torensma R
    Oncoimmunology, 2013-04-01;2(5):e24271.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Interleukin-27 induces a STAT1/3- and NF-kappaB-dependent proinflammatory cytokine profile in human monocytes.
    Authors: Guzzo C, Che Mat NF, Gee K
    J. Biol. Chem., 2010-06-02;285(32):24404-11.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Flow-cytometric analysis of cytokine production in human paracoccidioidomycosis.
    Authors: Mamoni RL, Blotta MH
    Cytokine, 2006-10-11;35(3):207-16.
    Species: Human
    Sample Types: Whole Blood
    Applications: Flow Cytometry

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