Human CXCL5/ENA-78 Antibody

Catalog #: MAB2541 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB2541
MAB2541-SP

Detection of Human CXCL5/ENA‑78 by Western Blot.

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All CXCL5/ENA-78 Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CXCL5/ENA-78 Antibody Summary

Species Reactivity

Human

Specificity

Detects human CXCL5/ENA‑78 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human CXCL1, 2, 3, 4, 6, 7, 8, 9, 10, 11, 12, 13, 16, recombinant mouse CXCL1, 2, 4, 6, 7, 9, 10, 11, 12, 15, 16, recombinant rat CXCL5, and recombinant porcine CXCL8 is observed.

Source

Monoclonal Mouse IgG₁ Clone # 33103

Purification

Protein A or G purified from ascites

Immunogen

E. coli-derived recombinant human CXCL5/ENA-78
Ala37-Asn114
Accession # P42830

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human CXCL5/ENA‑78 by Western Blot. Western blot shows lysates of conditioned media (CM) and lysates from A549 human lung carcinoma cell line untreated (-) or treated (+) with human IL-1 beta for 24 hours. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human CXCL5/ENA-78 Monoclonal Antibody (Catalog # MAB2541) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for CXCL5/ENA-78 at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CXCL5/ENA-78

CXCL5, also known as epithelial cell-derived neutrophil-activating peptide (ENA-78), is an 8 kDa proinflammatory member of the CXC subfamily of chemokines. Its Glu-Leu-Arg (ELR) motif confers angiogenic properties and distinguishes it from ELR-CXC chemokines which are angiostatic (1‑3). Human CXCL5 shares 57% amino acid (aa) sequence identity with mouse and rat CXCL5. Among other human ELR⁺ chemokines, it shares 77% aa sequence identity with CXCL6/GCP-2 and 35%‑51% with CXCL1/GRO alpha, CXCL2/GRO beta, CXCL3/GRO gamma, CXCL7/NAP-2, and CXCL8/IL-8. Inflammatory stimulation upregulates CXCL5 production in multiple hematopoietic cell types, fibroblasts, endothelial cells, and vascular smooth muscle cells. In vivo, CXCL5 is elevated at sites of inflammation and pulmonary fibrosis where it promotes neutrophil infiltration and activation as well as angiogenesis (3‑6). Its upregulation contributes to increased vascularization, tumor growth, and metastasis in many cancers (6‑9). Full length CXCL5 (78 aa) is trimmed at the N-terminus by cathepsin G and chymotrypsin to ENA-74 (74 aa) and ENA-70 (70 aa), with the shortened forms showing increased potency relative to full length CXCL5 (10, 11). CXCL5 exerts its effects primarily through interactions with CXCR2 (6, 12). It also binds duffy antigen receptor for chemokines (DARC), which can limit CXCR2-mediated responses (13, 14).

References

Strieter, R.M. et al. (2005) Cytokine Growth Factor Rev. 16:593.
Balestrieri, M.L. et al. (2008) Cardiovasc. Res. 78:250.
Walz, A.. et al. (1991) J. Exp. Med. 174:1355.
Strieter, R.M. et al. (1992) Immunol. Invest. 21:549.
Koch, A.E. et al. (1994) J. Clin. Invest. 94:1012.
Begley, L.A. et al. (2008) Neoplasia 10:244.
Vandercappellen, J. et al. (2008) Cancer Lett. 267:226.
Arenberg, D.A. et al. (1998) J. Clin. Invest. 102:465.
Miyazaki, H. et al. (2006) Cancer Res. 66:4279.
Wuyts, A. et al. (1999) Eur. J. Biochem. 260:421.
Nufer, O. et al. (1999) Biochemistry 38:636.
Ahuja, S.K. and P.M. Murphy (1996) J. Biol. Chem. 271:20545.
Kashiwazaki, M. et al. (2003) Int. Immunol. 15:1219.
Horton, L.W. et al. (2007) Cancer Res. 67:9791.

Entrez Gene IDs

6374 (Human)

Alternate Names

chemokine (C-C motif) ligand 5; C-X-C motif chemokine 5; CXCL5; CXCL5/ENA-78; ENA78; ENA-78; ENA-78(1-78); Epithelial-derived neutrophil-activating protein 78; Neutrophil-activating peptide ENA-78; neutrophil-activating protein 78; SCYB5ENA78