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Human CXCL5/ENA-78 Antibody

Catalog #: MAB254
7 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB254-SP
MAB254-500
MAB254-100
CXCL5/ENA‑78 in Human Colon Cancer Tissue.
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Product Details
Citations (7)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CXCL5/ENA-78 Antibody Summary

Species Reactivity
Human
Specificity
Detects human CXCL5/ENA‑78 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) CXCL1, 2, 3, 8, 10, or recombinant mouse CXCL2 is observed.
Source
Monoclonal Mouse IgG1 Clone # 33160
Purification
Protein A or G purified from ascites
Immunogen
E. coli-derived recombinant human CXCL5/ENA-78
Ala37-Asn114 (predicted)
Accession # P42830
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human CXCL5/ENA‑78 (Catalog # 254-XB)
under non-reducing conditions only
Immunohistochemistry
5-25 µg/mL
Immersion fixed paraffin-embedded sections of human colon cancer tissue
Neutralization
Measured by its ability to neutralize CXCL5/ENA‑78-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR2. The Neutralization Dose (ND50) is typically 3-15 µg/mL in the presence of 0.03 µg/mL Recombinant Human CXCL5/ENA‑78.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry View Larger

CXCL5/ENA‑78 in Human Colon Cancer Tissue. CXCL5/ENA‑78 was detected in immersion fixed paraffin-embedded sections of human colon cancer tissue using Mouse Anti-Human CXCL5/ENA‑78 Monoclonal Antibody (Catalog # MAB254) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in epithelial cells and secreted protein. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Neutralization Chemotaxis Induced by CXCL5/ENA‑78 and Neutral-ization by Human CXCL5/ENA‑78 Antibody. View Larger

Chemotaxis Induced by CXCL5/ENA‑78 and Neutral-ization by Human CXCL5/ENA‑78 Antibody. Recombinant Human CXCL5/ENA-78 (Catalog # 254-XB) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CXCL5/ENA-78 (0.03 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CXCL5/ENA-78 Monoclonal Antibody (Catalog # MAB254). The ND50is typically 3-15 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CXCL5/ENA-78

CXCL5, also known as epithelial cell-derived-neutrophil-activating-peptide (ENA78), is an 8 kDa proinflammatory member of the CXC subfamily of chemokines. Its Glu-Leu-Arg (ELR) motif confers angiogenic properties and distinguishes it from ELR- CXC chemokines which are angiostatic. Human CXCL5 shares 57% amino acid (aa) sequence identity with mouse and rat CXCL5. Among other human ELR+ chemokines, it shares 77% aa sequence identity with CXCL6/GCP2 and 35%‑51% with CXCL1/GRO alpha, CXCL2/GRO beta, CXCL3/GRO gamma, CXCL7/NAP2, and CXCL8/IL8. Inflammatory stimulation upregulates CXCL5 production in multiple hematopoietic cell types, fibroblasts, endothelial cells, and vascular smooth muscle cells. In vivo, CXCL5 is elevated at sites of inflammation and pulmonary fibrosis where it promotes neutrophil infiltration and activation as well as angiogenesis. Its upregulation contributes to increased vascularization, tumor growth, and metastasis in many cancers. Full length CXCL5 (78 aa) is trimmed at the N-terminal end by Cathepsin G and chymotrypsin to ENA74 (74 aa) and ENA70 (70 aa), with the shortened forms showing increased potency relative to full length CXCL5. CXCL5 exerts its effects primarily through interactions with CXCR2. It also binds DARC, a decoy chemokine receptor which can limit CXCR2-mediated responses.

Entrez Gene IDs
6374 (Human)
Alternate Names
chemokine (C-C motif) ligand 5; C-X-C motif chemokine 5; CXCL5; CXCL5/ENA-78; ENA78; ENA-78; ENA-78(1-78); Epithelial-derived neutrophil-activating protein 78; Neutrophil-activating peptide ENA-78; neutrophil-activating protein 78; SCYB5ENA78

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Citations for Human CXCL5/ENA-78 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. CXCL5 suppression recovers neovascularization and accelerates wound healing in diabetes mellitus
    Authors: Chen, C;Lin, LY;Chen, JW;Chang, TT;
    Cardiovascular diabetology
    Species: Human
    Sample Types: Protein, Whole Cells
    Applications: Western Blot, Neutralization
  2. CRISPR/Cas9-mediated deletion of Interleukin-30 suppresses IGF1 and CXCL5 and boosts SOCS3 reducing prostate cancer growth and mortality
    Authors: C Sorrentino, L D'Antonio, SL Ciummo, C Fieni, L Landuzzi, F Ruzzi, S Vespa, P Lanuti, LV Lotti, PL Lollini, E Di Carlo
    Journal of hematology & oncology, 2022-10-13;15(1):145.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  3. CXCL5 expression in tumor tissues is associated with poor prognosis in patients with pancreatic cancer
    Authors: B Wu, J Wang, X Wang, M Zhu, F Chen, Y Shen, Z Zhong
    Oncology Letters, 2020-09-18;20(5):257.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  4. CXCL5 secreted from adipose tissue-derived stem cells promotes cancer cell proliferation
    Authors: Y Zhao, X Zhang, H Zhao, J Wang, Q Zhang
    Oncol Lett, 2017-12-05;15(2):1403-1410.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  5. Podocytes regulate neutrophil recruitment by glomerular endothelial cells via IL-6-mediated crosstalk.
    Authors: Kuravi S, McGettrick H, Satchell S, Saleem M, Harper L, Williams J, Rainger G, Savage C
    J Immunol, 2014-05-28;193(1):234-43.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  6. CXCL5 stimulation of RANK ligand expression in Paget's disease of bone.
    Authors: Sundaram K, Rao D, Ries W, Reddy S
    Lab Invest, 2013-02-25;93(4):472-9.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Development
  7. Adipose tissue-derived stem cells secrete CXCL5 cytokine with chemoattractant and angiogenic properties.
    Authors: Zhang H, Ning H, Banie L, Wang G, Lin G, Lue TF, Lin CS
    Biochem. Biophys. Res. Commun., 2010-10-27;402(3):560-4.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization

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