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Human CXCL6/GCP-2 Antibody

Catalog #: MAB333
3 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB333
MAB333-SP
Chemotaxis Induced by CXCL6/GCP‑2 and Neutral-ization by Human CXCL6/ GCP‑2 Antibody.
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Citations (3)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CXCL6/GCP-2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human CXCL6/GCP‑2 in ELISAs and Western blots. In sandwich immunoassays, less than 0.04% cross-reactivity with recombinant human (rh) NAP-2, rhGRO alpha, rhGRO beta, rhBLC, rhENA-78, recombinant mouse (rm) CXCL6, rmMIP-2, rhIL-8, rhIP-10, rhMIG, rhSDF-1 alpha, and rhSDF-1 beta is observed.
Source
Monoclonal Mouse IgG1 Clone # 60910
Purification
Protein A or G purified from ascites
Immunogen
E. coli-derived recombinant human CXCL6/GCP-2
Val40-Asn114
Accession # P80162
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human CXCL6/GCP‑2 (Catalog # 333-GC)

Human CXCL6/GCP-2 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Human CXCL6/GCP‑2 Biotinylated Antibody (Catalog # BAF333)

Standard: Recombinant Human CXCL6/GCP-2 Protein (Catalog # 333-GC)

Neutralization
Measured by its ability to neutralize CXCL6/GCP‑2-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR2. The Neutralization Dose (ND50) is typically 8-30 μg/mL in the presence of 80 ng/mL Recombinant Human CXCL6/GCP‑2.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Chemotaxis Induced by CXCL6/GCP‑2 and Neutral-ization by Human CXCL6/ GCP‑2 Antibody. View Larger

Chemotaxis Induced by CXCL6/GCP‑2 and Neutral-ization by Human CXCL6/ GCP‑2 Antibody. Recombinant Human CXCL6/ GCP-2 (Catalog # 333-GC) induces chemotaxis in the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Under these conditions, chemotaxis elicited by CXCL6/GCP-2 is neutralized (green line) by increasing concentrations of Mouse Anti-Human CXCL6/GCP-2 Monoclonal Antibody (Catalog # MAB333). The ND50 is typically 8-30 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CXCL6/GCP-2

CXCL6, also known as Granulocyte Chemotactic Protein 2 (GCP-2), belongs to the CXC chemokine family. It functions as a neutrophil chemoattractant through interactions with CXCR2.

Entrez Gene IDs
6372 (Human)
Alternate Names
chemokine (C-X-C motif) ligand 6 (granulocyte chemotactic protein 2); Chemokine alpha 3; CKA-3Granulocyte chemotactic protein 2; CXCL6; GCP-2; GCP2member 6 (granulocytechemotactic protein 2); GCP-2Small-inducible cytokine B6; Small inducible cytokine subfamily B (Cys-X-Cys), member b

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Citations for Human CXCL6/GCP-2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Metronomic chemotherapy prevents therapy-induced stromal activation and induction of tumor-initiating cells
    Authors: Kelvin K Tsai
    J. Exp. Med., 2016-11-23;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  2. GCP-2/CXCL6 synergizes with other endothelial cell-derived chemokines in neutrophil mobilization and is associated with angiogenesis in gastrointestinal tumors.
    Authors: Penninckx F, Ectors N
    Exp. Cell Res., 2005-02-15;303(2):331-42.
    Species: Human
    Sample Types: Recombinant Protein, Whole Tissue
    Applications: IHC-P, Western Blot
  3. Disease modification and symptom relief in osteoarthritis using a mutated GCP ‐2/ CXCL6 chemokine
    Authors: Sara Caxaria, Nikolaos Kouvatsos, Suzanne E Eldridge, Mario Alvarez‐Fallas, Anne‐Sophie Thorup, Daniela Cici et al.
    EMBO Molecular Medicine

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