Human CXCL6/GCP‑2 Biotinylated Antibody

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BAF333
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Human CXCL6/GCP‑2 Biotinylated Antibody Summary

Species Reactivity
Human
Specificity
Detects human CXCL6/GCP-2 in ELISAs and Western blots. In sandwich immunoassays, less than 0.04% cross-reactivity with recombinant human (rh) NAP‑2, rhGRO alpha, rhGRO beta, rhBLC, rhENA-78, recombinant mouse (rm) CXCL6, rmMIP‑2, rhIL‑8, rhIP‑10, rhMIG, and rhSDF‑1 beta is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human CXCL6/GCP-2 (R&D Systems, Catalog # 333-GC)
Val40-Asn114
Accession # P80162
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human CXCL6/GCP‑2 (Catalog # 333-GC)

Human CXCL6/GCP-2 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Detection (Matched Antibody Pair)
0.1-0.4 µg/mL 

Use in combination with:

Capture Reagent: Human CXCL6/GCP‑2 Antibody (Catalog # MAB333)

Standard: Recombinant Human CXCL6/GCP-2 Protein (Catalog # 333-GC)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CXCL6/GCP-2

GCP-2 (granulocyte chemotactic protein-2) also known as CXCL6, is a CXC chemokine initially isolated as a neutrophil chemoattractant from the MG-63 osteosarcoma cell line. Among human CXC chemokines, GCP-2 is most closely related to ENA-78 (78% amino acid (aa) sequence identity in the mature peptide region and 86% identity in the signal sequence). The structure and sequence of the genes for human GCP-2 and ENA-78 also exhibit close similarity suggesting the two genes may have originated from a gene duplication. LIX (LPS-induced CXC chemokine) was initially cloned as a gene induced by LPS in mouse fibroblasts. The predicted LIX protein sequence is identical to a previously purified mouse protein designated mouse GCP-2 based on its amino sequence similarity (60% sequence identity) to human GCP-2. Mouse GCP-2/LIX is also 54% identical with human ENA-78 at the amino acid sequence level.

Human GCP-2 cDNA encodes a propeptide of 114 amino acid residues with a predicted 37 aa residue signal peptide and 77 aa residue mature protein. Several forms of natural GCP‑2 have been isolated from MG-63 conditioned media, indicating that GCP-2 undergoes limited processing at both the N- and C-termini. Human GCP-2 is a primary response gene whose induction by cytokines is attenuated by dexamethasone.

Human GCP-2 and mouse GCP-2/LIX have been shown to chemoattract and activate neutrophils, but not eosinophils and monocytes. It is likely that GCP-2 activities are mediated via the human or mouse CXCR2.

References
  1. Proost, P. et al. (1993) J. Immunol. 150:1000.
  2. Smith, J.B. and H.R. Herschman (1995) J. Biol. Chem. 270:16756.
  3. Rovai, L.E. et al. (1997) J. Immunol. 158:5257.
  4. Wuyts, A. et al. (1997) J. Immunol. 157:1736.
Entrez Gene IDs
6372 (Human)
Alternate Names
chemokine (C-X-C motif) ligand 6 (granulocyte chemotactic protein 2); Chemokine alpha 3; CKA-3Granulocyte chemotactic protein 2; CXCL6; GCP-2; GCP2member 6 (granulocytechemotactic protein 2); GCP-2Small-inducible cytokine B6; Small inducible cytokine subfamily B (Cys-X-Cys), member b

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