Human CXCR1/IL-8RA Antibody
Human CXCR1/IL-8RA Antibody Summary
Met1-Leu350
Accession # AAA59159
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
Detection of CXCR1/IL‑8 RA in Human Blood Granulocytes by Flow Cytometry. Human peripheral blood granulocytes were stained with Mouse Anti-Human CXCR1/IL-8 RA Monoclonal Antibody (Catalog # MAB330, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).
Chemotaxis Induced by CXCL8/IL‑8 and Neutralization by Human CXCR1/IL‑8 RA Antibody. Recombinant Human CXCL8/IL-8 (Catalog # 208-IL) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR1 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CXCL8/IL-8 (1 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CXCR1/IL-8 RA Monoclonal Antibody (Catalog # MAB330). The ND50 is typically 0.4-2.0 µg/mL.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CXCR1/IL-8RA
The human C-X-C chemokine IL-8 is a potent neutrophil chemotactic and activating factor. Two distinct G protein-linked cell surface receptors, known as IL-8 RA (type I or CXCR1) and IL-8 RB (type II or CXCR2), can interact with the IL-8 molecule. These two receptors share 77% amino acid homology. CXCR1 expression has been documented on neutrophils, monocytes, and a small population of T cells.
Product Datasheets
Citations for Human CXCR1/IL-8RA Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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The RNA-binding protein YBX1 regulates epidermal progenitors at a posttranscriptional level
Authors: E Kwon, K Todorova, J Wang, R Horos, KK Lee, VA Neel, GL Negri, PH Sorensen, SW Lee, MW Hentze, A Mandinova
Nat Commun, 2018-04-30;9(1):1734.
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IL8 derived from macrophages inhibits CD8+ T-cell function by downregulating TIM3 expression through IL8-CXCR2 axis in patients with advanced colorectal cancer
Authors: Zhao, C;Wang, D;Li, Z;Zhang, Z;Xu, Y;Liu, J;Lei, Q;Han, D;Huo, Y;Liu, S;Li, L;Zhang, Y;
International immunopharmacology
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Matrix Metalloproteinase 9 Induced in Esophageal Squamous Cell Carcinoma Cells via Close Contact with Tumor-Associated Macrophages Contributes to Cancer Progression and Poor Prognosis
Authors: Tsukamoto, S;Koma, YI;Kitamura, Y;Tanigawa, K;Azumi, Y;Miyako, S;Urakami, S;Hosono, M;Kodama, T;Nishio, M;Shigeoka, M;Yokozaki, H;
Cancers
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
The Chemokine Receptor CCR3 Is Potentially Involved in the Homing of Prostate Cancer Cells to Bone: Implication of Bone-Marrow Adipocytes
Authors: Adrien Guérard, Victor Laurent, Gaëlle Fromont, David Estève, Julia Gilhodes, Edith Bonnelye et al.
International Journal of Molecular Sciences
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Induced organoids derived from patients with ulcerative colitis recapitulate colitic reactivity
Authors: SK Sarvestani, S Signs, B Hu, Y Yeu, H Feng, Y Ni, DR Hill, RC Fisher, S Ferrandon, RK DeHaan, J Stiene, M Cruise, TH Hwang, X Shen, JR Spence, EH Huang
Nature Communications, 2021-01-11;12(1):262.
Species: Human
Sample Types: Organoid
Applications: IHC -
Mesothelial Cell HIF1alpha Expression Is Metabolically Downregulated by Metformin to Prevent Oncogenic Tumor-Stromal Crosstalk
Authors: PC Hart, HA Kenny, N Grassl, KM Watters, LM Litchfield, F Coscia, I Blaženovi?, L Ploetzky, O Fiehn, M Mann, E Lengyel, IL Romero
Cell Rep, 2019-12-17;29(12):4086-4098.e6.
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization -
Dynamic Transcriptome-Proteome Correlation Networks Reveal Human Myeloid Differentiation and Neutrophil-Specific Programming
Authors: AJ Hoogendijk, F Pourfarzad, CEM Aarts, ATJ Tool, IH Hiemstra, L Grassi, M Frontini, AB Meijer, M van den Bi, TW Kuijpers
Cell Rep, 2019-11-19;29(8):2505-2519.e4.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
The Synthetic Dipeptide Pidotimod Shows a Chemokine-Like Activity through CXC Chemokine Receptor 3 (CXCR3)
Authors: F Caccuri, A Bugatti, S Corbellini, S Roversi, A Zani, P Mazzuca, S Marsico, A Caruso, C Giagulli
Int J Mol Sci, 2019-10-24;20(21):.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Mammalian diaphanous-related formin 1 (mDia1) coordinates mast cell migration and secretion through its actin-nucleating activity
Authors: Ofir Klein, Rebecca A. Krier-Burris, Pia Lazki-Hagenbach, Yaara Gorzalczany, Yang Mei, Peng Ji et al.
Journal of Allergy and Clinical Immunology
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Development and application of a high-content virion display human GPCR array
Authors: Guan-Da Syu, Shih-Chin Wang, Guangzhong Ma, Shuang Liu, Donna Pearce, Atish Prakash et al.
Nature Communications
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IL-8/CXCR1/2 signalling promotes tumor cell proliferation, invasion and vascular mimicry in glioblastoma
Authors: I Sharma, A Singh, F Siraj, S Saxena
J. Biomed. Sci., 2018-08-08;25(1):62.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Altered monocyte differentiation and macrophage polarization patterns in patients with breast cancer
Authors: CH Hung, FM Chen, YC Lin, ML Tsai, SL Wang, YC Chen, YT Chen, MF Hou
BMC Cancer, 2018-04-03;18(1):366.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Role of autophagy in the HIV-1 matrix protein p17-driven lymphangiogenesis
Authors: P Mazzuca, S Marsico, K Schulze, S Mitola, MC Pils, C Giagulli, CA Guzman, A Caruso, F Caccuri
J. Virol., 2017-07-27;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Proteomic profiling of peripheral blood neutrophils identifies two inflammatory phenotypes in stable COPD patients
Authors: ALT Loi, S Hoonhorst, C van Aalst, J Langereis, V Kamp, S Sluis-Eisi, N Ten Hacken, JW Lammers, L Koenderman
Respir. Res., 2017-05-22;18(1):100.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Mycobacteria Manipulate G-Protein-Coupled Receptors to Increase Mucosal Rac1 Expression in the Lungs
Authors: Nader Alaridah
J Innate Immun, 2016-12-24;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization -
Autocrine signaling is a key regulatory element during osteoclastogenesis
Authors: Paul Kopesky, Kerstin Tiedemann, Dahlia Alkekhia, Christoph Zechner, Bjorn Millard, Birgit Schoeberl et al.
Biology Open
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The IL-8/CXCR1 axis is associated with cancer stem cell-like properties and correlates with clinical prognosis in human pancreatic cancer cases.
Authors: Chen, Lianyu, Fan, Jie, Chen, Hao, Meng, Zhiqiang, Chen, Zhen, Wang, Peng, Liu, Luming
Sci Rep, 2014-08-01;4(0):5911.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
CXCL1 inhibits airway smooth muscle cell migration through the decoy receptor Duffy antigen receptor for chemokines.
Authors: Al-Alwan L, Chang Y, Rousseau S, Martin J, Eidelman D, Hamid Q
J Immunol, 2014-06-30;193(3):1416-26.
Species: Human
Sample Types: Cell Lysates, Whole Cells
Applications: Neutralization, Western Blot -
Chinese herbal medicine suppresses invasion-promoting capacity of cancer-associated fibroblasts in pancreatic cancer.
Authors: Chen, Lianyu, Qu, Chao, Chen, Hao, Xu, Litao, Qi, Qi, Luo, Jianmin, Wang, Kun, Meng, Zhiqiang, Chen, Zhen, Wang, Peng, Liu, Luming
PLoS ONE, 2014-04-29;9(4):e96177.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Orphan receptor GPR15/BOB is up-regulated in rheumatoid arthritis.
Authors: Cartwright A, Schmutz C, Askari A, Kuiper J, Middleton J
Cytokine, 2014-03-22;67(2):53-9.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Simian immunodeficiency virus and human immunodeficiency virus type 1 matrix proteins specify different capabilities to modulate B cell growth.
Authors: Caccuri, Francesc, Giagulli, Cinzia, Reichelt, Joachim, Martorelli, Debora, Marsico, Stefania, Bugatti, Antonell, Barone, Ines, Rusnati, Marco, Guzman, Carlos A, Dolcetti, Riccardo, Caruso, Arnaldo
J Virol, 2014-03-12;88(10):5706-17.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
CXCL1/CXCL8 (GROalpha/IL-8) in human diabetic ketoacidosis plasma facilitates leukocyte recruitment to cerebrovascular endothelium in vitro.
Authors: Omatsu T, Cepinskas G, Clarson C, Patterson E, Alharfi I, Summers K, Couraud P, Romero I, Weksler B, Fraser D
Am J Physiol Endocrinol Metab, 2014-03-11;306(9):E1077-84.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
The endothelin-integrin axis is involved in macrophage-induced breast cancer cell chemotactic interactions with endothelial cells.
Authors: Chen C, Chen L, Hsu Y, Liu K, Fan C, Huang T
J Biol Chem, 2014-02-18;289(14):10029-44.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Hypoxia promotes the proliferation of cervical carcinoma cells through stimulating the secretion of IL-8.
Authors: Liu L, Xie F, Chang K, Li M, Meng Y, Wang X, Li H, Li D, Yu J
Int J Clin Exp Pathol, 2014-01-15;7(2):575-83.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
Expansion of activated regulatory T cells by myeloid-specific chemokines via an alternative pathway in CSF of bacterial meningitis patients.
Authors: Shi G, Han J, Liu G, Hao Y, Ma Y, Li T, Wu X, Zhang H, Liu Y, Wang B, Kong Y, Zhou J, Zeng H
Eur J Immunol, 2013-12-02;44(2):420-30.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Glioblastoma stem cells are regulated by interleukin-8 signaling in a tumoral perivascular niche.
Authors: Infanger, David W, Cho, YouJin, Lopez, Brina S, Mohanan, Sunish, Liu, S Chris, Gursel, Demirkan, Boockvar, John A, Fischbach, Claudia
Cancer Res, 2013-10-11;73(23):7079-89.
Species: Human
Sample Types: Whole Cells
Applications: Functional Assay -
Targeting CXCR1/2 Significantly Reduces Breast Cancer Stem Cell Activity and Increases the Efficacy of Inhibiting HER2 via HER2-Dependent and -Independent Mechanisms
Authors: Jagdeep K. Singh, Gillian Farnie, Nigel J. Bundred, Bruno M Simões, Amrita Shergill, Göran Landberg et al.
Clinical Cancer Research
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Bronchoepithelial Expression of CXCR1 and CXCR2 Does Not Facilitate Transepithelial Migration of Neutrophils
Authors: Christian Schulz, Benjamin Stoelcker, Brigitte Ruhland, Nico Jentsch, Andreas Steege
Respiration
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Adipocytes promote ovarian cancer metastasis and provide energy for rapid tumor growth.
Authors: Nieman KM, Kenny HA, Penicka CV, Ladanyi A, Buell-Gutbrod R, Zillhardt MR, Romero IL, Carey MS, Mills GB, Hotamisligil GS, Yamada SD, Peter ME, Gwin K, Lengyel E
Nat. Med., 2011-10-30;17(11):1498-503.
Species: Human
Sample Types: In Vivo
Applications: In Vivo -
Gene Expression of Pro-Inflammatory Cytokines and Neuropeptides in Diabetic Wound Healing
Authors: Leena Pradhan, Xuemei Cai, Szuhuei Wu, Nicholas D. Andersen, Michelle Martin, Junaid Malek et al.
Journal of Surgical Research
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Enrichment of Foxp3+ CD4 regulatory T cells in migrated T cells to IL-6- and IL-8-expressing tumors through predominant induction of CXCR1 by IL-6.
Authors: Eikawa S, Ohue Y, Kitaoka K
J. Immunol., 2010-11-03;185(11):6734-40.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
IL8 and CXCL13 are potent chemokines for the recruitment of human neural precursor cells across brain endothelial cells.
Authors: Weiss N, Deboux C, Chaverot N, Miller F, Baron-Van Evercooren A, Couraud P, Cazaubon S
J Neuroimmunol, 2010-04-18;223(1):131-4.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Reduced expression of chemoattractant receptors by polymorphonuclear leukocytes in Hyper IgE Syndrome patients.
Authors: Mintz R, Garty BZ, Meshel T, Marcus N, Katanov C, Cohen-Hillel E, Ben-Baruch A
Immunol. Lett., 2009-12-11;130(1):97-106.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Spatiotemporal expression of chemokines and chemokine receptors in experimental anti-myeloperoxidase antibody-mediated glomerulonephritis.
Authors: van der Veen BS, Petersen AH, Belperio JA, Satchell SC, Mathieson PW, Molema G, Heeringa P
Clin. Exp. Immunol., 2009-10-01;158(1):143-53.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
CXCR1 and CXCR2 enhances human melanoma tumourigenesis, growth and invasion.
Authors: Singh S, Nannuru KC, Sadanandam A, Varney ML, Singh RK
Br. J. Cancer, 2009-04-28;100(10):1638-46.
Species: Human
Sample Types: Cell Lysates, Whole Cells
Applications: ICC, Western Blot -
Potential role of high-mobility group box 1 in cystic fibrosis airway disease.
Authors: Rowe SM, Jackson PL, Liu G, Hardison M, Livraghi A, Solomon GM, McQuaid DB, Noerager BD, Gaggar A, Clancy JP, O'Neal W, Sorscher EJ, Abraham E, Blalock JE
Am. J. Respir. Crit. Care Med., 2008-07-24;178(8):822-31.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Expression of CXCL8, CXCR1, and CXCR2 in neurons and glial cells of the human and rabbit retina.
Authors: Goczalik I, Ulbricht E, Hollborn M, Raap M, Uhlmann S, Weick M, Pannicke T, Wiedemann P, Bringmann A, Reichenbach A, Francke M
Invest. Ophthalmol. Vis. Sci., 2008-06-14;49(10):4578-89.
Species: Human, Rabbit
Sample Types: Whole Tissue
Applications: IHC-Fr -
A novel proteolytic cascade generates an extracellular matrix-derived chemoattractant in chronic neutrophilic inflammation.
Authors: Gaggar A, Jackson PL, Noerager BD, O'Reilly PJ, McQuaid DB, Rowe SM, Clancy JP, Blalock JE
J. Immunol., 2008-04-15;180(8):5662-9.
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization -
Shear-induced capping of L-selectin on the neutrophil surface during centrifugation.
Authors: Lee D, King MR
J. Immunol. Methods, 2007-09-12;328(1):97-105.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Role of interleukin-8 and growth-regulated oncogene-alpha in the chemotactic migration of all-trans retinoic acid-treated promyelocytic leukemic cells toward alveolar epithelial cells.
Authors: Tsai WH, Hsu HC, Lin CC, Ho CK, Kou YR
Crit. Care Med., 2007-03-01;35(3):879-85.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Increased levels of neutrophil-activating peptide-2 in acute coronary syndromes: possible role of platelet-mediated vascular inflammation.
Authors: Smith C, Damas JK, Otterdal K, ØIe E, Sandberg WJ, Yndestad A, Waehre T, Scholz H, Endresen K, Olofsson PS, Halvorsen B, Gullestad L, Froland SS, Hansson GK, Aukrust P
J. Am. Coll. Cardiol., 2006-09-27;48(8):1591-9.
Species: Human
Sample Types: Whole Cells, Whole Tissue
Applications: IHC-Fr, IHC-P, Neutralization -
Cytokine and chemokine profiles in multiple myeloma; significance of stromal interaction and correlation of IL-8 production with disease progression.
Authors: Kline M, Donovan K, Wellik L, Lust C, Jin W, Moon-Tasson L, Xiong Y, Witzig TE, Kumar S, Rajkumar SV, Lust JA
Leuk. Res., 2006-08-01;31(5):591-8.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Increased expression of Th2-associated chemokines in bullous pemphigoid disease. Role of eosinophils in the production and release of these chemokines.
Authors: Gounni Abdelilah S, Wellemans V, Agouli M, Guenounou M, Hamid Q, Beck LA, Lamkhioued B
Clin. Immunol., 2006-06-16;120(2):220-31.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Chemokine receptor expression in rat adjuvant-induced arthritis.
Authors: Haas CS, Martinez RJ, Attia N, Haines GK, Campbell PL, Koch AE
Arthritis Rheum., 2005-12-01;52(12):3718-30.
Species: Rat
Sample Types: Whole Tissue
Applications: IHC -
IL-17 enhances the net angiogenic activity and in vivo growth of human non-small cell lung cancer in SCID mice through promoting CXCR-2-dependent angiogenesis.
Authors: Numasaki M, Watanabe M, Suzuki T, Takahashi H, Nakamura A, McAllister F, Hishinuma T, Goto J, Lotze MT, Kolls JK, Sasaki H
J. Immunol., 2005-11-01;175(9):6177-89.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Neutralization -
The activation of IL-8 receptors in cultured guinea pig Muller glial cells is modified by signals from retinal pigment epithelium.
Authors: Malgorzata Goczalik I, Raap M, Weick M, Milenkovic I, Heidmann J, Enzmann V, Wiedemann P, Reichenbach A, Francke M
J. Neuroimmunol., 2005-04-01;161(1):49-60.
Species: Guinea Pig
Sample Types: Whole Cells
Applications: ICC -
Evasion from NK cell immunity by MHC class I chain-related molecules expressing colon adenocarcinoma.
Authors: Doubrovina ES, Doubrovin MM, Vider E, Sisson RB, O'Reilly RJ, Dupont B, Vyas YM
J. Immunol., 2003-12-15;171(12):6891-9.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Possible link between unique chemokine and homing receptor expression at diagnosis and relapse location in a patient with childhood T-ALL.
Authors: Annels NE, Willemze AJ, van der Velden VH, Faaij CM, van Wering E, Sie-Go DM, Egeler RM, van Tol MJ, Révész T
Blood, 2003-12-04;103(7):2806-8.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Expression of functional CCR and CXCR chemokine receptors in podocytes.
Authors: Huber TB, Reinhardt HC, Exner M, Burger JA, Kerjaschki D, Saleem MA, Pavenstadt H
J. Immunol., 2002-06-15;168(12):6244-52.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-Fr -
Interleukin 8 Receptor Deficiency Confers Susceptibility to Acute Experimental Pyelonephritis and May Have a Human Counterpart
Authors: Björn Frendéus, Gabriela Godaly, Long Hang, Diana Karpman, Ann-Charlotte Lundstedt, Catharina Svanborg
J. Exp. Med
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