Human CXCR5 PerCP-conjugated Antibody

Catalog #: FAB190C Datasheet / COA / SDS

Clone 51505 was used by HLDA to establish CD designation

Catalog #	Availability	Size / Price	Qty
FAB190C-025
FAB190C-100

Detection of CXCR5 in Human Blood Lymphocytes by Flow Cytometry.

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All CXCR5 Antibodies

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Human CXCR5 PerCP-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Stains human CXCR5 transfectants but not the parental cell lines in flow cytometry. Does not cross-react with human CXCR2, CXCR3, or CXCR4 transfectants.

Source

Monoclonal Mouse IgG_2B Clone # 51505

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

NS0 mouse myeloma cell line transfected with human CXCR5
Met1-Phe372
Accession # P32302

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

PerCP (Peridinin-chlorophyll Protein Complex) (Excitation= 482 and 564 nm, Emission= 675 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of CXCR5 in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD19 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB4867F) and either (A) Mouse Anti-Human CXCR5 PerCP-conjugated Monoclonal Antibody (Catalog # FAB190C) or (B) Mouse IgG_2BPerCP Isotype Control (Catalog # IC0041C). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: CXCR5

CXCR5, also known as BLR-1, is a 7 transmembrane domain protein expressed on B cells. CXCR5 mediates B cell migration following binding of CXCL13.

Entrez Gene IDs

643 (Human); 12145 (Mouse); 29363 (Rat)

Alternate Names

BLR1; BLR1MDR-15; Burkitt lymphoma receptor 1; Burkitt lymphoma receptor 1, GTP binding protein (chemokine (C-X-C motif)receptor 5); Burkitt lymphoma receptor 1, GTP-binding protein; CD185 antigen; CD185; chemokine (C-X-C motif) receptor 5; C-X-C chemokine receptor type 5; CXCR5; CXC-R5; MDR15CXCR-5; MGC117347; Monocyte-derived receptor 15