Human CXCR6 Antibody

Catalog #: MAB6991 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB6991-100
MAB6991-SP

Detection of CXCR6 in Human PBMCs Stimulated to Induce Th1 Cells by Flow Cytometry.

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All CXCR6 Antibodies

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Human CXCR6 Antibody Summary

Species Reactivity

Human

Specificity

Detects human CXCR6 in direct ELISAs.

Source

Monoclonal Mouse IgG_2A Clone # 1010910

Purification

Protein A or G purified from ascites

Immunogen

HEK293 human embryonic kidney cell line transfected with human CXCR6
Met1-Leu342
Accession # O00574

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of CXCR6 in Human PBMCs Stimulated to Induce Th1 Cells by Flow Cytometry. Human CD3⁺peripheral blood mononuclear cells (PBMCs) cultured with plate bound anti-Human CD3 and CD28 Antibodies, Anti-Human IL-4 Polyclonal Antibody (Catalog # AB-204-NA; 20 µg/mL) and 10 ng/mL Recombinant Human IL-12 (Catalog # 219-IL) for 5 days to induce Th1 cell development were stained with (A) Mouse Anti-Human CXCR6 Monoclonal Antibody (Catalog # MAB6991) or (B) Isotype control antibody (Catalog # MAB003) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B) and Mouse Anti-Human CD4 APC-conjugated Monoclonal Antibody (Catalog # FAB3791A). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CXCR6

CXCR6, also known as BONZO, STRL33, TYMSTR, is the receptor for the C-X-C chemokine CXCL16. CXCR6 is a G protein coupled protein receptor (GPCR) expressed on activated T cells and tumor-infiltrating lymphocytes, and it has been identified as an entry co-receptor used by HIV-1 and SIV to enter target cells, in conjunction with CD4.

Entrez Gene IDs

10663 (Human); 80901 (Mouse); 100124593 (Rat)

Alternate Names

Bonzo; BONZOG-protein coupled receptor bonzo; CD186 antigen; CD186; CDw186; chemokine (C-X-C motif) receptor 6; C-X-C chemokine receptor type 6; CXCR6; CXC-R6; CXCR-6; G protein-coupled receptor; STRL33; STRL33G-protein coupled receptor STRL33; TYMSTRCD186