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Human CXCR6 Antibody

Catalog #: MAB699
26 Citations Datasheet / COA / SDS
Clone 56811 was used by HLDA to establish CD designation
Catalog # Availability Size / Price Qty
MAB699-100
MAB699-SP
Detection of CXCR6 in Human PBMCs by Flow Cytometry.
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Product Details
Citations (26)
FAQs
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CXCR6 Antibody Summary

Species Reactivity
Human
Specificity
Stains human CXCR6 transfectants but not irrelevant transfectants.
Source
Monoclonal Mouse IgG2B Clone # 56811
Purification
Protein A or G purified from ascites
Immunogen
NS0 mouse myeloma cell line transfected with human CXCR6
Met1-Leu342
Accession # O00574
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-reported
Strauss-Albee, D.M. et al. (2015) Sci. Transl. Med. 7: 297ra115. Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of CXCR6 antibody in Human PBMCs antibody by Flow Cytometry. View Larger

Detection of CXCR6 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) either (A) treated with 20 ng/mL Recombinant Human IL-2 (Catalog # 202-IL) for 6 days or (B) untreated were stained with Mouse Anti-Human CXCR6 Monoclonal Antibody (Catalog # MAB699) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B) and Mouse Anti-Human CD3e PE-conjugated Monoclonal Antibody (Catalog # FAB100P). Quadrant markers were set based on control antibody staining (Catalog # MAB0041). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry Detection of CXCR6 antibody in HEK293 Human cell line transfected with Human CXCR6 and eGFP antibody by Flow Cytometry. View Larger

Detection of CXCR6 in HEK293 Human cell line transfected with Human CXCR6 and eGFP by Flow Cytometry. HEK293 Human cell line transfected with either (A) Human CXCR6 or (B) irrelevant transfectant and eGFP, was stained with Mouse Anti-Human CXCR6 Monoclonal Antibody (Catalog # MAB699) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB0041). View our protocol for Staining Membrane-associated Proteins.

Immunocytochemistry/ Immunofluorescence Detection of Human CXCR6 by Immunocytochemistry/Immunofluorescence View Larger

Detection of Human CXCR6 by Immunocytochemistry/Immunofluorescence CXCR6 expression in tissues samples from LuCa patients(A) Representative images for CXCR6 tissue expression in lung tissues from non-neoplastic (n = 8), adenocarcinoma (n = 54) and squamous cell carcinoma (n = 24) were stained with isotype control or anti-CXCR6 antibodies. Brown (DAB) color shows CXCR6 staining. Images were captured using tissuefaxs cell analysis system from Tissuegnostics. (B) Immuno-intensities of CXCR6 were quantified using image analysis Aperio ImageScope v.6.25 software using ImageScope algorithm. ***p ≤ 0.0001 when compared between groups. Image collected and cropped by CiteAb from the following publication (https://www.oncotarget.com/lookup/doi/10.18632/oncotarget.3194), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CXCR6

CXCR6, also known as Bonzo and STRL33, is a G protein coupled chemokine receptor expressed on activated T cells and tumor-infiltrating lymphocytes. It binds soluble CXCL16 and membrane-anchored CXCL16 expressed on dendritic cells and macrophages.

Entrez Gene IDs
10663 (Human); 80901 (Mouse); 100124593 (Rat)
Alternate Names
Bonzo; BONZOG-protein coupled receptor bonzo; CD186 antigen; CD186; CDw186; chemokine (C-X-C motif) receptor 6; C-X-C chemokine receptor type 6; CXCR6; CXC-R6; CXCR-6; G protein-coupled receptor; STRL33; STRL33G-protein coupled receptor STRL33; TYMSTRCD186

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Citations for Human CXCR6 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

26 Citations: Showing 1 - 10
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  1. LILRB1-HLA-G axis defines a checkpoint driving natural killer cell exhaustion in tuberculosis
    Authors: Wang, J;Chai, Q;Lei, Z;Wang, Y;He, J;Ge, P;Lu, Z;Qiang, L;Zhao, D;Yu, S;Qiu, C;Zhong, Y;Li, BX;Zhang, L;Pang, Y;Gao, GF;Liu, CH;
    EMBO molecular medicine
    Species: Human
    Sample Types: Whole Cells
    Applications: CyTOF-reported
  2. MicroRNA-222 Regulates Melanoma Plasticity
    Authors: MC Lionetti, F Cola, O Chepizhko, MR Fumagalli, F Font-Clos, R Ravasio, S Minucci, P Canzano, M Camera, G Tiana, S Zapperi, CAM Porta
    J Clin Med, 2020-08-08;9(8):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Evidence for a pathogenic role of extrafollicular, IL-10-producing CCR6+B helper T cells in systemic lupus erythematosus
    Authors: F Facciotti, P Larghi, R Bosotti, C Vasco, N Gagliani, C Cordiglier, S Mazzara, V Ranzani, E Rottoli, S Curti, A Penatti, B Karnani, Y Kobayashi, M Crosti, M Bombaci, JP van Hambur, G Rossetti, R Gualtierot, M Gerosa, S Gatti, S Torretta, L Pignataro, SW Tas, S Abrignani, M Pagani, F Grassi, PL Meroni, RA Flavell, J Geginat
    Proc. Natl. Acad. Sci. U.S.A., 2020-03-17;117(13):7305-7316.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Mesenchymal stem cell-derived CXCL16 promotes progression of gastric cancer cells by STAT3-mediated expression of Ror1
    Authors: T Ikeda, M Nishita, K Hoshi, T Honda, Y Kakeji, Y Minami
    Cancer Sci., 2020-02-25;111(4):1254-1265.
    Species: Human
    Sample Types: Tissue
    Applications: IHC
  5. Development and application of a high-content virion display human GPCR array
    Authors: Guan-Da Syu, Shih-Chin Wang, Guangzhong Ma, Shuang Liu, Donna Pearce, Atish Prakash et al.
    Nature Communications
  6. The Chemokine Receptor CXCR6 Evokes Reverse Signaling via the Transmembrane Chemokine CXCL16
    Authors: V Adamski, R Mentlein, R Lucius, M Synowitz, J Held-Feind, K Hattermann
    Int J Mol Sci, 2017-07-08;18(7):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  7. Chemoprophylaxis with sporozoite immunization in P. knowlesi rhesus monkeys confers protection and elicits sporozoite-specific memory T cells in the liver
    Authors: S Pichyangku, MD Spring, K Yongvanitc, U Kum-Arb, A Limsalakpe, R Im-Erbsin, R Ubalee, P Vanachayan, EJ Remarque, E Angov, PL Smith, DL Saunders
    PLoS ONE, 2017-02-09;12(2):e0171826.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. Glatiramer Acetate, Dimethyl Fumarate, and Monomethyl Fumarate Upregulate the Expression of CCR10 on the Surface of Natural Killer Cells and Enhance Their Chemotaxis and Cytotoxicity
    Authors: Zaidoon Al-Jaderi
    Front Immunol, 2016-10-19;7(0):437.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. Wnt5a-Ror2 signaling in mesenchymal stem cells promotes proliferation of gastric cancer cells by activating CXCL16-CXCR6 axis.
    Authors: Gosuke Takiguchi, Michiru Nishita, Kana Kurita, Yoshihiro Kakeji, Yasuhiro Minami
    Cancer Science, 2016-02-09;0(0):1349-7006.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  10. CXCR6 expression in non-small cell lung carcinoma supports metastatic process via modulating metalloproteinases
    Authors: Hina Mir, Rajesh Singh, Goetz H. Kloecker, James W. Lillard, Shailesh Singh
    Oncotarget
  11. CXCL16 and CXCR6 are coexpressed in human lung cancer in vivo and mediate the invasion of lung cancer cell lines in vitro.
    Authors: Hu, Weidong, Liu, Yue, Zhou, Wenhui, Si, Lianlian, Ren, Liang
    PLoS ONE, 2014-06-04;9(6):e99056.
    Species: Human
    Sample Types: Whole Cells, Whole Tissue
    Applications: ICC, IHC-P
  12. In vitro migration capacity of human adipose tissue-derived mesenchymal stem cells reflects their expression of receptors for chemokines and growth factors
    Authors: Sun Jin Baek, Sung Keun Kang, Jeong Chan Ra
    Experimental and Molecular Medicine
  13. Expression and function of CXCL16 in a novel model of gout
    Authors: Jeffrey H. Ruth, Monica D. Arendt, M. Asif Amin, Salahuddin Ahmed, Hubert Marotte, Bradley J. Rabquer et al.
    Arthritis & Rheumatism
  14. Chemokine-enhanced chemotaxis of lymphangioleiomyomatosis cells with mutations in the tumor suppressor TSC2 gene.
    Authors: Pacheco-Rodriguez G, Kumaki F, Steagall WK, Zhang Y, Ikeda Y, Lin JP, Billings EM, Moss J
    J. Immunol., 2009-02-01;182(3):1270-7.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  15. Expression of the CXCR6 on polymorphonuclear neutrophils in pancreatic carcinoma and in acute, localized bacterial infections.
    Authors: Gaida MM, Gunther F, Wagner C, Friess H, Giese NA, Schmidt J, Hansch GM, Wente MN
    Clin. Exp. Immunol., 2008-09-05;154(2):216-23.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  16. Expression and potential function of the CXC chemokine CXCL16 in pancreatic ductal adenocarcinoma.
    Authors: Wente MN, Gaida MM, Mayer C, Michalski CW, Haag N, Giese T, Felix K, Bergmann F, Giese NA, Friess H
    Int. J. Oncol., 2008-08-01;33(2):297-308.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  17. Overexpression of CXCL16 and its receptor CXCR6/Bonzo promotes growth of human schwannomas.
    Authors: Held-Feindt J, Rehmke B, Mentlein R, Hattermann K, Knerlich F, Hugo HH, Ludwig A, Mehdorn HM
    Glia, 2008-05-01;56(7):764-74.
    Species: Human
    Sample Types: Cell Lysates, Whole Tissue
    Applications: IHC, Western Blot
  18. Vitamin A metabolites induce gut-homing FoxP3+ regulatory T cells.
    Authors: Kang SG, Lim HW, Andrisani OM, Broxmeyer HE, Kim CH
    J. Immunol., 2007-09-15;179(6):3724-33.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  19. Expression and role of CCR6/CCL20 chemokine axis in pulmonary sarcoidosis.
    Authors: Facco M, Baesso I, Miorin M, Bortoli M, Cabrelle A, Boscaro E, Gurrieri C, Trentin L, Zambello R, Calabrese F, Cassatella MA, Semenzato G, Agostini C
    J. Leukoc. Biol., 2007-07-05;82(4):946-55.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr, IHC-P
  20. CD molecules 2006--human cell differentiation molecules.
    Authors: Zola H, Swart B, Banham A, Barry S, Beare A, Bensussan A, Boumsell L, D Buckley C, Buhring HJ, Clark G, Engel P, Fox D, Jin BQ, Macardle PJ, Malavasi F, Mason D, Stockinger H, Yang X
    J. Immunol. Methods, 2006-12-04;319(1):1-5.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  21. The majority of human peripheral blood CD4+CD25highFoxp3+ regulatory T cells bear functional skin-homing receptors.
    Authors: Hirahara K, Liu L, Clark RA, Yamanaka K, Fuhlbrigge RC, Kupper TS
    J. Immunol., 2006-10-01;177(7):4488-94.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  22. TLR4-NOX4-AP-1 signaling mediates lipopolysaccharide-induced CXCR6 expression in human aortic smooth muscle cells.
    Authors: Patel DN, Bailey SR, Gresham JK, Schuchman DB, Shelhamer JH, Goldstein BJ, Foxwell BM, Stemerman MB, Maranchie JK, Valente AJ, Mummidi S, Chandrasekar B
    Biochem. Biophys. Res. Commun., 2006-07-13;347(4):1113-20.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  23. CXCL16-mediated cell recruitment to rheumatoid arthritis synovial tissue and murine lymph nodes is dependent upon the MAPK pathway.
    Authors: Ruth JH, Haas CS, Park CC, Amin MA, Martinez RJ, Haines GK, Shahrara S, Campbell PL, Koch AE
    Arthritis Rheum., 2006-03-01;54(3):765-78.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  24. Regulatory T cells can migrate to follicles upon T cell activation and suppress GC-Th cells and GC-Th cell-driven B cell responses.
    Authors: Lim HW, Hillsamer P, Kim CH
    J. Clin. Invest., 2004-12-01;114(11):1640-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  25. Possible link between unique chemokine and homing receptor expression at diagnosis and relapse location in a patient with childhood T-ALL.
    Authors: Annels NE, Willemze AJ, van der Velden VH, Faaij CM, van Wering E, Sie-Go DM, Egeler RM, van Tol MJ, Révész T
    Blood, 2003-12-04;103(7):2806-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  26. Unique subpopulations of CD56+ NK and NK-T peripheral blood lymphocytes identified by chemokine receptor expression repertoire.
    Authors: Campbell J, Qin S, Unutmaz D, Soler D, Murphy K, Hodge M, Wu L, Butcher E
    J Immunol, 2001-06-01;166(11):6477-82.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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