Human CXCR6 APC-conjugated Antibody
Human CXCR6 APC-conjugated Antibody Summary
Met1-Leu342
Accession # O00574
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of CXCR6 in HEK293 Human Cell Line Transfected with Human CXCR6 by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with human CXCR6 was stained with Mouse Anti-Human CXCR6 APC-conjugated Monoclonal Antibody (Catalog # FAB699A, filled histogram) or isotype control antibody (Catalog # IC0041A, open histogram). View our protocol for Staining Membrane-associated Proteins.
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Detection of CXCR6 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) either (A) untreated or (B) treated with 20 ng/mL Recombinant Human IL-2 (Catalog # 202-IL) for 9 days were stained with Mouse Anti-Human CXCR6 APC-conjugated Monoclonal Antibody (Catalog # FAB699A) and Mouse Anti-Human CD3e PE-conjugated Monoclonal Antibody (Catalog # FAB100P). Quadrant markers were set based on control antibody staining (Catalog # IC0041A). View our protocol for Staining Membrane-associated Proteins.
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Detection of CXCR6 by Flow Cytometry CXCR6 promotes BC cells migration and invasionA) CXCR6 was overexpressed in MCF-7 and MDA-231 BC cells by lentiviral system. CXCR6 -positive cells were then sorted by flow cytometry. Histogram displays the ratio of CXCR6 positive expression of MCF-7CXCR6, MDA-231CXCR6 and their respective control cells. B) Scratch wound healing assay shows that overexpression of CXCR6 enhanced cell migration in MDA-231 cells, but not very significant in MCF-7 cells. C) Matrigel invasion assay showed that overexpression of CXCR6 significantly enhanced cell invasion in both MCF-7 and MDA-231 cells (***P < 0.001). Experiments were performed 3 times; representative images of invaded cells are shown. Mean ± SD. D) Cytometry analysis showed CXCR6 had no effect on cell cycle. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/25909173), licensed under a CC-BY license. Not internally tested by R&D Systems.
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Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CXCR6
CXCR6, also known as Bonzo and STRL33, is a G protein coupled chemokine receptor expressed on activated T cells and tumor-infiltrating lymphocytes. It binds soluble CXCL16 and membrane-anchored CXCL16 expressed on dendritic cells and macrophages.
Product Datasheets
Citations for Human CXCR6 APC-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
6
Citations: Showing 1 - 6
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CXCR4 inhibition in human pancreatic and colorectal cancers induces an integrated immune response
Authors: D Biasci, M Smoragiewi, CM Connell, Z Wang, Y Gao, JED Thaventhir, B Basu, L Magiera, TI Johnson, L Bax, A Gopinathan, C Isherwood, FA Gallagher, M Pawula, I Hudecova, D Gale, N Rosenfeld, P Barmpounak, EC Popa, R Brais, E Godfrey, F Mir, FM Richards, DT Fearon, T Janowitz, DI Jodrell
Proc Natl Acad Sci U S A, 2020-10-30;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Resident memory T cells are a cellular reservoir for HIV in the cervical mucosa
Authors: J Cantero-Pé, J Grau-Expós, C Serra-Pein, DA Rosero, L Luque-Ball, A Astorga-Ga, J Castellví, T Sanhueza, G Tapia, B Lloveras, MA Fernández, JG Prado, JM Solé-Seden, A Tarrats, C Lecumberri, L Mañalich-B, C Centeno-Me, V Falcó, MJ Buzon, M Genescà
Nat Commun, 2019-10-18;10(1):4739.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Role of CXCL13 and CCL20 in the recruitment of B cells to inflammatory foci in chronic arthritis
Authors: E Armas-Gonz, MJ Domínguez-, A Díaz-Martí, M Arce-Franc, J Castro-Her, G Danelon, V Hernández-, S Bustabad-R, A Cantabrana, M Uguccioni, F Díaz-Gonzá
Arthritis Res. Ther., 2018-06-07;20(1):114.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
IL-27 in human secondary lymphoid organs attracts myeloid dendritic cells and impairs HLA class I-restricted antigen presentation.
Authors: Morandi, Fabio, Di Carlo, Emma, Ferrone, Soldano, Petretto, Andrea, Pistoia, Vito, Airoldi, Irma
J Immunol, 2014-02-19;192(6):2634-42.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Chemokine responsiveness of CD4+ CD25+ regulatory and CD4+ CD25- T cells from atopic and nonatopic donors.
Authors: Ahern D, Lloyd CM, Robinson DS
Allergy, 2009-02-07;64(8):1121-9.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Expression of the CXCR6 on polymorphonuclear neutrophils in pancreatic carcinoma and in acute, localized bacterial infections.
Authors: Gaida MM, Gunther F, Wagner C, Friess H, Giese NA, Schmidt J, Hansch GM, Wente MN
Clin. Exp. Immunol., 2008-09-05;154(2):216-23.
Species: Human
Sample Types: Whole Cells, Whole Tissue
Applications: Flow Cytometry, IHC-P
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Gated on lymphocyte FSC-A and SSC-A populations. Singlets were further gated on by FSC-A/FSC-H doublet exclusion.
Buffer: 2% Human Serum, 0.5 mM EDTA in PBS
Dilution: 1/100
Cells were stained for 10 minutes in room temperature followed by three washes.
Specificity: Specific
Sensitivity: Reasonably sensitive
Buffer: PBS 2.5% FBS
Dilution: 1/10
