Home / CXCR7/RDC-1 / Human CXCR7/RDC-1 PE-conjugated Antibody

Human CXCR7/RDC-1 PE-conjugated Antibody

Catalog #: FAB42271P
9 Citations Datasheet / COA / SDS

Discontinued Product

FAB42271P has been discontinued.
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Detection of CXCR7/RDC‑1 in Human Blood Monocytes by Flow Cytometry.
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Citations (9)
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Human CXCR7/RDC-1 PE-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human CXCR7/RDC‑1 in direct ELISAs. In flow cytometry, reacts specifically with five distinct human CXCR7 transfectants, but does not react with their respective parental lines or mouse CXCR7 transfectants. In flow cytometry, also reacts with monocytes expressing CXCR7, but does not react with MCF-7 cells which have been reported to have surface-expressing CXCR7 using clone 11G8. Due to the conflicting reports published, use of clone 358426 may result in an underestimation of CXCR7 expression on certain cell types.
Source
Monoclonal Mouse IgG2A Clone # 358426
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with human CXCR7/RDC‑1
Met1-Lys362 (Gly131Ser)
Accession # AAA62370
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of CXCR7/RDC-1 antibody in Human Blood Monocytes antibody by Flow Cytometry. View Larger

Detection of CXCR7/RDC‑1 in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Mouse Anti-Human CXCR7/ RDC-1 PE-conjugated Monoclonal Antibody (Catalog # FAB42271P, filled histogram) or isotype control antibody (Catalog # IC003P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CXCR7/RDC-1

The G protein-coupled receptor, RDC1, belongs to a subgroup of chemokine receptors and has been designated CXCR7. CXCR7 can bind with high-affinity to CXCL12/SDF-1 and CXCL11/I-TAC. It is also a co-receptor for several HIV and SIV strains. In their N-termini and extracellular loops 1, 2, and 3, human and mouse CXCR7 share 84%, 100%, 96% and 86% amino acid sequence identity, respectively. Reports of mRNA levels and/or protein expression (as assessed using anti‑CXCR7, clone 9C4) (1, 2) indicate that CXCR7 occurs on a wide variety of tissues and cells including monocytes, B cells, T cells and mature dendritic cells. In contrast, based on ligand binding analysis and receptor level (as assessed using anti‑CXCR7, clone 11G8), surface expression of CXCR7 was reported to be restricted to tumor cells, activated endothelial cells, fetal liver cells, and few other cell types (3). The basis of these inconsistent observations is not known but may be attributed to cell context and the use of different antibodies that may recognize different epitopes.

References
  1. Balabanian, K. et al. (2005) J. Biol. Chem. 280:35760.
  2. Infantino, S. et al. (2006) J. Immunol. 176:2197.
  3. Burns, J.M. et al. (2006) J. Exp. Med. 203:2201.
Entrez Gene IDs
57007 (Human); 12778 (Mouse); 84348 (Rat)
Alternate Names
ACKR3; chemokine (C-X-C motif) receptor 7; Chemokine orphan receptor 1CMKOR1C-X-C chemokine receptor type 7; CMKOR1; CXCR7; CXC-R7; CXCR-7; G protein-coupled receptor; GPR159; GPR159G-protein coupled receptor 159; RDC-1; RDC1G-protein coupled receptor RDC1 homolog

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Citations for Human CXCR7/RDC-1 PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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  1. Hepatic stellate cells express functional CXCR4: role in stromal cell-derived factor-1alpha-mediated stellate cell activation
    Authors: Feng Hong, Ana Tuyama, Ting Fang Lee, Johnny Loke, Ritu Agarwal, Xin Cheng et al.
    Hepatology
  2. Decursin inhibits tumor progression in head and neck squamous cell carcinoma by downregulating CXCR7 expression in�vitro
    Authors: M Joo, JB Heo, S Kim, N Kim, HJ Jeon, Y An, GY Song, JM Kim, HJ Lee
    Oncology reports, 2021-12-27;47(2):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Decursin inhibits tumor growth, migration, and invasion in gastric cancer by down-regulating CXCR7 expression
    Authors: S Kim, JE Kim, N Kim, M Joo, MW Lee, HJ Jeon, H Ryu, IC Song, GY Song, HJ Lee
    Am J Cancer Res, 2019-09-01;9(9):2007-2018.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Mutational Analysis of Atypical Chemokine Receptor 3 (ACKR3/CXCR7) Interaction with its Chemokine Ligands CXCL11 and CXCL12
    Authors: Nikolaus Heveker
    J. Biol. Chem., 2016-11-14;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Targeting chemokine receptor CXCR7 inhibits glioma cell proliferation and mobility.
    Authors: Liu Y, Carson-Walter E, Walter K
    Anticancer Res, 2015-01-01;35(1):53-64.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Chemokine receptor CXCR7 is a functional receptor for CXCL12 in brain endothelial cells.
    Authors: Liu Y, Carson-Walter E, Walter K
    PLoS ONE, 2014-08-01;9(8):e103938.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. GMP-compliant isolation and large-scale expansion of bone marrow-derived MSC.
    Authors: Fekete N, Rojewski M, Furst D, Kreja L, Ignatius A, Dausend J, Schrezenmeier H
    PLoS ONE, 2012-08-14;7(8):e43255.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. Expression profiles of novel cell surface molecules on B-cell subsets and plasma cells as analyzed by flow cytometry.
    Authors: Llinas L, Lazaro A, de Salort J, Matesanz-Isabel J, Sintes J, Engel P
    Immunol. Lett., 2010-10-15;134(2):113-21.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. Severe congenital neutropenia resulting from G6PC3 deficiency with increased neutrophil CXCR4 expression and myelokathexis.
    Authors: McDermott DH, De Ravin SS, Jun HS
    Blood, 2010-07-08;116(15):2793-802.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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