Human Cystatin E/M Antibody Summary
Arg29-Met149
Accession # Q15828
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human Cystatin E/M by Western Blot. Western blot shows lysates of human skin tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Cystatin E/M Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1286) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Cystatin E/M at approximately 13 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Detection of Human Cystatin E/M by Simple WesternTM. Simple Western lane view shows lysates of human skin tissue, loaded at 0.2 mg/mL. A specific band was detected for Cystatin E/M at approximately 13 kDa (as indicated) using 50 µg/mL of Goat Anti-Human Cystatin E/M Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1286) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Cystatin E/M
Cystatin E/M encoded by the CST6 gene is a member of family 2 of the cystatin superfamily (1, 2). It inhibits papain and cathepsin B, two of the cysteine proteases. Its mRNA was found in many tissues by the two groups who did initial cloning (1, 2). However, its protein was found only in skin and sweat glands by a third group (3). In addition to being a cysteine protease inhibitor, cystatin E/M is also a substrate for transglutaminases (3). It is required for viability and for correct formation of cornified layers in the epidermis and hair follicles, as ichq mice, with a null mutation in the cystatin E/M gene, have defects in epidermal cornification and die between 5 and 12 days of age (4). Cystatin E/M expression and function may not be limited to cutaneous epithelia. For example, it is found in rat brain and is induced during neuronal cell differentiation (5).
- Sotiropoulou, G. et al. (1997) J. Biol. Chem. 272:903.
- Ni, J. et al. (1997) J. Biol. Chem. 272:10853.
- Zeeuwen, P.L. et al. (2001) J. Invest. Dermatol. 116:693.
- Zeeuwen, P.L. et al. (2002) Hum. Mol. Genet. 11:2867.
- Hong, J. et al. (2002) J. Neurochem. 81:922.
Product Datasheets
Citations for Human Cystatin E/M Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 6
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Glycosylation is important for legumain localization and processing to active forms but not for cystatin E/M inhibitory functions
Authors: NN Lunde, MH Haugen, KB Bodin Lars, I Damgaard, SJ Pettersen, R Kasem, W Rut, M Drag, M Poreba, HT Johansen, R Solberg
Biochimie, 2017-05-17;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Correction: Nuclear Legumain Activity in Colorectal Cancer
Authors: Mads H. Haugen, Harald T. Johansen, Solveig J. Pettersen, Rigmor Solberg, Klaudia Brix, Kjersti Flatmark et al.
PLoS ONE
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Nuclear Legumain Activity in Colorectal Cancer
Authors: Mads H. Haugen, Harald T. Johansen, Solveig J. Pettersen, Rigmor Solberg, Klaudia Brix, Kjersti Flatmark et al.
PLoS ONE
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Differential secretome analysis reveals CST6 as a suppressor of breast cancer bone metastasis.
Cell Res., 2012-06-12;22(9):1356-73.
Species: Human
Sample Types: Cell Lysates, Whole Cells, Whole Tissue
Applications: IHC, Neutralization, Western Blot -
Cystatin E/M suppresses legumain activity and invasion of human melanoma.
Authors: Briggs JJ, Haugen MH, Johansen HT, Riker AI, Abrahamson M, Fodstad O, Maelandsmo GM, Solberg R
BMC Cancer, 2010-01-15;10(1):17.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Invasion suppressor cystatin E/M (CST6): high-level cell type-specific expression in normal brain and epigenetic silencing in gliomas.
Authors: Qiu J, Ai L, Ramachandran C, Yao B, Gopalakrishnan S, Fields CR, Delmas AL, Dyer LM, Melnick SJ, Yachnis AT, Schwartz PH, Fine HA, Brown KD, Robertson KD
Lab. Invest., 2008-07-07;88(9):910-25.
Species: Human
Sample Types: Whole Cells
Applications: ICC
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