Human Cystatin F Antibody

Catalog #: MAB1889 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB1889
MAB1889-SP

All Cystatin F Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Cystatin F Antibody Summary

Species Reactivity

Human

Specificity

Detects human Cystatin F in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) Cystatin A, B, C, D, E/M, S, recombinant mouse (rm) Cystatin/Stefin Homolog, rhFetuin A, rhFetuin B, rhHPRG, rhKininogen, or rhKininostatin is observed.

Source

Monoclonal Mouse IgG_2B Clone # 292103

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Cystatin F
Gly20-His145
Accession # O76096

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human Cystatin F (Catalog # 1889-PI) under non-reducing conditions only

Immunoprecipitation

25 µg/mL

Conditioned cell culture medium spiked with Recombinant Human Cystatin F (Catalog # 1889-PI), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cystatin F

Cystatin F, also known as leukocystatin and CMAP (Cystatin-like Metastasis-Associated Protein), is a member of the cystatin superfamily (1‑3). Cystatin F is selectively expressed by hematopoietic cells and may be a biomarker for both liver metastasis and inflammatory lung disorders (3, 4). As a cysteine protease inhibitor, it shows selectivity towards cathepsin L and legumain (1, 2, 5). Compared to other secreted cystatins including C, D, E/M, S, SA and SN, which contain two intra disulfide bonds, cystatin F has two extra Cys residues that may be involved in inter disulfide bonds. Indeed, rhCystatin F showed disulfide bond-linked dimer formation, which was also the case for an E. coli expressed fusion protein containing mature human cystatin F and glutathione S-transferase (2).

References