Human Cystatin SN Biotinylated Antibody

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BAF1285
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Human Cystatin SN Biotinylated Antibody Summary

Species Reactivity
Human
Specificity
Detects human Cystatin SN in Western blots. In Western blots, approximately 30% cross-reactivity with recombinant human (rh) Cystatin SA is observed, 15% cross-reactivity with rhCystatin S is observed, 5% cross‑reactivity with rhCystatin D is observed, and less than 1% cross‑reactivity with rhCystatin A, rhCystatin B, rhCystatin C, rhCystatin E/M, and rhCystatin F is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Cystatin SN
Trp21-Ser141
Accession # P01037
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human Cystatin SN (Catalog # 1285-PI)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cystatin SN

Cystatin SN is a member of family 2 of the cystatin superfamily (1). Together with cystatins S and SA, it is produced by the salivary gland and secreted largely in the submandibular/sublingual saliva (2). Cystatin SN inhibits members of the papain family including cathepsins B, C, H and L (3).

References
  1. Abrahamson, M. (1994) Methods Enzymol. 244:685.
  2. Baron, A.C. et al. (1999) Oral Dis. 5:344.
  3. Baron, A. et al. (1999) Oral Dis. 5:234.
Entrez Gene IDs
1469 (Human)
Alternate Names
CST1; cystain-SA-I; cystatin 1; cystatin SA-I; Cystatin SN; cystatin-1; cystatin-SN; Salivary cystatin-SA-1; type 2 family

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