Human Cytokeratin 17/19 Antibody

Detection of Human Cytokeratin 17/19 by Western Blot. Western Blot shows lysates of HeLa human cervical epithelial carcinoma cell line and HCC827 human non-small cell lung cancer cell line. PVDF membrane was probed with 2 µg/ml of Mouse Anti-Human Cytokeratin 17/19 Monoclonal Antibody (Catalog # MAB11568) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for Cytokeratin 17 at approximately 48 kDa and for Cytokeratin 19 at approximately 41 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Detection of Cytokeratin 17/19 in Human Prostate. Cytokeratin 17/19 was detected in immersion fixed paraffin-embedded sections of human prostate using Mouse Anti-Human Cytokeratin 17/19 Monoclonal Antibody (Catalog # MAB11568) at 5 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001) or the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the membrane of basal cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of Cytokeratin 17/19 in Human Squamous Cell Carcinoma. Cytokeratin 17/19 was detected in immersion fixed paraffin-embedded sections of human squamous cell carcinoma using Mouse Anti-Human Cytokeratin 17/19 Monoclonal Antibody (Catalog # MAB11568) at 5 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001) or the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the membrane. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of Human Cytokeratin 17/19 by Simple Western^TM. Simple Western shows lysates of HeLa human cervical epithelial carcinoma cell line and MDA‑MB‑468 human breast cancer cell line, loaded at 0.5 mg/ml. Specific bands were detected for Cytokeratin 17 at approximately 53kDa and for Cytokeratin 19 at approximately 51kDa (as indicated) using 20 µg/mL of Mouse Anti-Human Cytokeratin 17/19 Monoclonal Antibody (Catalog # MAB11568). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.