Human Cytokeratin 8 Antibody Summary
Applications
Lane, E.B. et al. (1985) Ann. NY Acad. Sci. 455:241. This application was not tested by R&D Systems.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Cytokeratin 8 in MCF‑7 Human Cell Line. Cytokeratin 8 was detected in immersion fixed MCF-7 human breast cancer cell line using Mouse Anti-Human Cytokeratin 8 Monoclonal Antibody (Catalog # MAB3165) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
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Cytokeratin 8 in Human Colon Cancer Tissue. Cytokeratin 8 was detected in immersion fixed paraffin-embedded sections of human colon cancer tissue using Mouse Anti-Human Cytokeratin 8 Monoclonal Antibody (Catalog # MAB3165) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membranes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Cytokeratin 8
Cytokeratin 8 is one of the first cytokeratins expressed in the embryo and exists in adult tissues as a heterodimer with Cytokeratin 18. Cytokeratin 8 is primarily found in the non-squamous epithelia and is expressed in the majority of adenocarcinomas and ductal carcinomas.
Product Datasheets
Citations for Human Cytokeratin 8 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 4
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Visualising SARS-CoV-2 infection of the lung in deceased COVID-19 patients
Authors: Van Slambrouck, J;Khan, M;Verbeken, E;Choi, S;Geudens, V;Vanluyten, C;Feys, S;Vanhulle, E;Wollants, E;Vermeire, K;De Fays, C;Aversa, L;Kaes, J;Van Raemdonck, D;Vos, R;Vanaudenaerde, B;De Hertogh, G;Wauters, E;Wauters, J;Ceulemans, LJ;Mombaerts, P;
EBioMedicine
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Expansion-enhanced super-resolution radial fluctuations enable nanoscale molecular profiling of pathology specimens
Authors: D Kylies, M Zimmermann, F Haas, M Schwerk, M Kuehl, M Brehler, J Czogalla, LC Hernandez, L Konczalla, Y Okabayashi, J Menzel, I Edenhofer, S Mezher, H Aypek, B Dumoulin, H Wu, S Hofmann, O Kretz, N Wanner, NM Tomas, S Krasemann, M Glatzel, C Kuppe, R Kramann, B Banjanin, RK Schneider, C Urbschat, P Arck, N Gagliani, M van Zandvo, T Wiech, F Grahammer, PJ Sáez, MN Wong, S Bonn, TB Huber, VG Puelles
Nature nanotechnology, 2023-04-10;18(4):336-342.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
TGF?1 in fibroblasts-derived exosomes promotes epithelial-mesenchymal transition of ovarian cancer cells
Authors: W Li, X Zhang, J Wang, M Li, C Cao, J Tan, D Ma, Q Gao
Oncotarget, 2017-10-06;8(56):96035-96047.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Visualizing in deceased COVID-19 patients how SARS-CoV-2 attacks the respiratory and olfactory mucosae but spares the olfactory bulb
Authors: Khan M, Yoo SJ, Clijsters M et al.
Cell
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Antibody was printed on custom arrays and incubated with fluorescently labeled human EDTA plasma
