Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Immunoprecipitation

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all Cytosol Nonspecific Dipeptidase (CNDP2)/CPGL Primary Antibodies »

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Cytosol Nonspecific Dipeptidase (CNDP2)/CPGL
Ala2-Asp475
Accession # Q96KP4

Specificity

Detects human Cytosol Nonspecific Dipeptidase (CNDP2)/CPGL in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human CPGL-2 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Applications for Human Cytosol Nonspecific Dipeptidase (CNDP2)/CPGL Antibody

Application
Recommended Usage

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Cytosol Nonspecific Dipeptidase (CNDP2)/CPGL (Catalog # 3560-ZN), see our available Western blot detection antibodies

Western Blot

0.1 µg/mL
Sample: Recombinant Human Cytosol Nonspecific Dipeptidase (CNDP2)/CPGL (Catalog # 3560-ZN)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Cytosol Nonspecific Dipeptidase (CNDP2)/CPGL

The human CNDP2 gene encodes cytosol non-specific dipeptidase, a member of the M20 family of metalloproteases (1, 2). Also known as glutamate carboxypeptidase-like protein 1 (CPGL), tissue carnosinase or peptidase A, CNDP2 is ubiquitously expressed throughout human tissues. Unlike CNDP1, CNDP2 has broad substrate specificity for dipeptides with free amino and carboxyl groups. The purified recombinant human CNDP2 migrates as three bands in SDS-PAGE under reducing conditions that correspond to the full length protein (54 kDa), the N-terminal fragment (33 kDa) and the C-terminal fragment (22 kDa). The initiator Met1 may be removed and Ala2 may be acetylated based on Accession # Q96KP4.

References

  1. Teufel, M. et al. (2003) J. Biol. Chem. 278:6521.
  2. Woessner, J. (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, A.J. et al.) pp.1020, Academic Press, San Diego.

Alternate Names

CN2, CNDP2, CPGL, PEPA

Entrez Gene IDs

55748 (Human)

Gene Symbol

CNDP2

UniProt

Q96KP4

Additional Cytosol Nonspecific Dipeptidase (CNDP2)/CPGL Products

Product Documents for Human Cytosol Nonspecific Dipeptidase (CNDP2)/CPGL Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human Cytosol Nonspecific Dipeptidase (CNDP2)/CPGL Antibody

For research use only

Related Research Areas

Metalloproteases and Regulators

Citations for Human Cytosol Nonspecific Dipeptidase (CNDP2)/CPGL Antibody

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