Human Cytosolic beta-Glucosidase/GBA3 Antibody

Catalog #: MAB59691 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB59691
MAB59691-SP

Cytosolic beta ‑Glucosidase/GBA3 in HeLa Human Cell Line.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Cytosolic beta-Glucosidase/GBA3 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Cytosolic beta ‑Glucosidase/GBA3 in direct ELISAs.

Source

Monoclonal Mouse IgG_2B Clone # 728702

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Cytosolic beta ‑Glucosidase/GBA3
Thr13-Leu469
Accession # Q9H227

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Immunocytochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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Cytosolic beta ‑Glucosidase/GBA3 in HeLa Human Cell Line. Cytosolic beta -Glucosidase/GBA3 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human Cytosolic beta -Glucosidase/GBA3 Monoclonal Antibody (Catalog # MAB59691) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cytosolic beta-Glucosidase/GBA3

There are three beta-glucosidases (GBA) in human genome. GBA1 endodes a lysosomal membrane protein that cleaves the beta-glucosidic linkage of glucosylceramide (1). GBA2 encodes a microsomal beta-glucosidase that catalyzes the hydrolysis of bile acid 3-O-glucosides (2). GBA3 is a cytosolic beta-glucosidase and is predominantly expressed in liver. GBA3 efficiently hydrolyzes beta-D-glucoside and beta-D-galactoside, but not any known physiological beta-glycoside, suggesting that it may be involved in detoxification of plant glycosides (3). GBA3 also has significant neutral glycosylceramidase activity, suggesting that it may be involved in a nonlysosomal catabolic pathway of glucosylceramide metabolism (4). At the protein level, GBA3 shows significant homology (>40%) with Klotho protein that is known for its association with aging process (3, 4).

References

Tybulewicz, V.L. et al. (1992) Nature 357:407.
Matern, H. et al. (2001) J. Biol. Chem. 276:37929.
de Graaf, M. et al. (2001) Biochem. J. 356:907.
Hayashi, Y. et al. (2007) J. Biol. Chem. 282:30889.

Entrez Gene IDs

57733 (Human); 289687 (Rat)

Alternate Names

CBG; CBGL1; CBGL1GLUC; Cytosolic betaGlucosidase; Cytosolic beta-Glucosidase; Cytosolic beta-glucosidase-like protein 1; EC 3.2.1.21; GBA3; GLUC; glucosidase, beta, acid 3 (cytosolic); klotho-related protein; KLrP; MGC104276; MGC126878