Human Cytosolic Sulfotransferase 1A1/SULT1A1 Antibody

Catalog #: MAB5546 Datasheet / COA / SDS

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MAB5546
MAB5546-SP

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Detection of Human Cytosolic Sulfotransferase 1A1/SULT1A1 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Cytosolic Sulfotransferase 1A1/SULT1A1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Cytosolic Sulfotransferase 1A1/SULT1A1 in direct ELISAs and Western blots. In direct ELISAs, 100% cross-reactivity with recombinant human (rh) SULT1A3 and rhSULT1C4 and approximately 10% cross-reactivity with rhSULT1B1 is observed. No cross-reactivity with rhSULT1E1, 2A1, 2B1, or 4A1 is observed.

Source

Monoclonal Mouse IgG₁ Clone # 638708

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human Cytosolic Sulfotransferase 1A1/SULT1A1
Glu2-Leu295
Accession # P50225

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

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Applications

Recommended Concentration

Sample

Western Blot

0.5 µg/mL

See below

Immunohistochemistry

8-25 µg/mL

See below

Immunoprecipitation

25 µg/mL

Cell lysates spiked with Recombinant Human SULT1A1 (Catalog # 5546-ST), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Cytosolic Sulfotransferase 1A1/SULT1A1 by Western Blot. Western blot shows lysates of human liver tissue. PVDF Membrane was probed with 0.5 µg/mL of Human Cytosolic Sulfotransferase 1A1/SULT1A1 Monoclonal Antibody (Catalog # MAB5546) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Cytosolic Sulfotransferase 1A1/SULT1A1 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry

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Cytosolic Sulfotransferase 1A1/SULT1A1 in Human Brain. Cytosolic Sulfotransferase 1A1/SULT1A1 was detected in immersion fixed paraffin-embedded sections of human brain using Human Cytosolic Sulfotransferase 1A1/SULT1A1 Monoclonal Antibody (Catalog # MAB5546) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to neurons and glial cells (punctate). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cytosolic Sulfotransferase 1A1/SULT1A1

Cytosolic Sulfotransferase 1A1 (SULT1A1; also phenol sulfotransferase 1 and thermostable phenol sulfotransferase) is a 35 kDa enzyme belonging to the sulfotransferase 1 family of proteins. Human SULT1A1 is 295 amino acids (aa) in length. Human SULT1A1 shares 78% and 73% aa identity with rat and mouse SULT1A1, respectively. SULT1A1 exists as a homodimer and is expressed in liver, lung, adrenal gland, brain, platelets, and skin. Functionally, it catalyzes the sulfate conjugation of catecholamines, phenolic drugs and neurotransmitter. It also mediates the metabolic activation of carcinogenic N-hydroxyarylamines to DNA binding products and could participate as a modulating factor of cancer risk.

Entrez Gene IDs

6817 (Human); 20887 (Mouse); 83783 (Rat)

Alternate Names

Aryl sulfotransferase 1; Cytosolic Sulfotransferase 1A1; EC 2.8.2; EC 2.8.2.1; HAST1/HAST2; Phenol sulfotransferase 1; Phenol-sulfating phenol sulfotransferase 1; P-PST 1; P-PST; PST; ST1A1; ST1A3; STP1 ; STP1MGC5163; STPMGC131921; sulfotransferase 1A1; sulfotransferase family, cytosolic, 1A, phenol-preferring, member 1; SULT1A1; Thermostable phenol sulfotransferase; thermostable phenol sulfotransferase1; ts-PST; TSPST1