Human Cytosolic Sulfotransferase 2A1/SULT2A1 Antibody

Catalog # Availability Size / Price Qty
AF5828
AF5828-SP

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Detection of Human Cytosolic Sulfotransferase 2A1/ SULT2A1 by Western Blot.
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Human Cytosolic Sulfotransferase 2A1/SULT2A1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Cytosolic Sulfotransferase 2A1/SULT2A1 in direct ELISAs and Western blots. In direct ELISAs, less than 2% cross-reactivity with recombinant human (rh) SULT1A1, rhSULT1A3, rhSULT1B1, rhSULT1E1, and rhSULT4A1 is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human Cytosolic Sulfotransferase 2A1/SULT2A1
Ser2-Glu285
Accession # Q06520
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below
Immunocytochemistry
5-15 µg/mL
See below
Neutralization
Measured by its ability to neutralize Recombinant Human Cytosolic Sulfotransferase 2A1/SULT2A1 (8.33 µg/mL, Catalog # 5828-ST) transfer from the sulfate donor Adenosine 3'-phosphate 5'-phosphosulfate (PAPS, 33 μM) to Trans-Dehydroandrosterone (DHEA, 133 µM). The Neutralization Dose (ND50) is typically 35.9 µg/mL.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Cytosolic Sulfotransferase 2A1/ SULT2A1 antibody by Western Blot. View Larger

Detection of Human Cytosolic Sulfotransferase 2A1/ SULT2A1 by Western Blot. Western blot shows lysates of human liver tissue. PVDF Membrane was probed with 0.5 µg/mL of Human Cytosolic Sulfotransferase 2A1/ SULT2A1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5828) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Cytosolic Sulfotransferase 2A1/SULT2A1 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Immunocytochemistry Cytosolic Sulfotransferase 2A1/SULT2A1 antibody in HepG2 Human Cell Line by Immunocytochemistry (ICC). View Larger

Cytosolic Sulfotransferase 2A1/SULT2A1 in HepG2 Human Cell Line. Cytosolic Sulfotransferase 2A1/SULT2A1 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Sheep Anti-Human Cytosolic Sulfotransferase 2A1/SULT2A1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5828) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cytosolic Sulfotransferase 2A1/SULT2A1

Sulfotransferase 2A1 (SULT2A1), also called DHEA-ST (DHEA/bile acid sulfotransferase), is a 35 kDa member of the cytosolic sulfotransferase family, which are Phase II drug-metabolizing enzymes that catalyze the sulfation of many endogenous and xenobiotic substrates. SULT2A1 is primarily expressed in liver and adrenal tissues. It sulfates steroids and bile acids, playing roles in maintaining cholesterol and bile acid homeostasis by increasing cholesterol catabolism and preventing toxicity from bile acid accumulation. Human SULT2A1 shares 63-65% amino acid identity with mouse and rat SULT2A1.

Entrez Gene IDs
6822 (Human); 20859 (Mouse); 24912 (Rat)
Alternate Names
alcohol/hydroxysteroid sulfotransferase; Cytosolic Sulfotransferase 2A1; Dehydroepiandrosterone sulfotransferase; DHEAS; DHEA-ST; DHEA-STbile salt sulfotransferase; EC 2.8.2.14; HST; hSTa; Hydroxysteroid Sulfotransferase; ST2; ST2A1; ST2A3; STDbile-salt sulfotranasferase 2A1; Sulfotransferase 2A1; sulfotransferase family, cytosolic, 2A, dehydroepiandrosterone(DHEA)-preferring, member 1; SULT2A1

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