Human DAP10/HCST Antibody

Catalog #: MAB9786 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB9786-100
MAB9786-SP

Detection of DAP10/HCST in U937 cell line by Flow Cytometry.

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All DAP10/HCST Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human DAP10/HCST Antibody Summary

Species Reactivity

Human

Specificity

Detects human DAP10/HCST in direct ELISAs.

Source

Monoclonal Mouse IgG_2A Clone # 982129

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Synthetic peptide containing human DAP10/HCST
Accession # Q9UBK5

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Intracellular Staining by Flow Cytometry

0.25 µg/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry

Detection of DAP10/HCST antibody in U937 cell line antibody by Flow Cytometry.

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Detection of DAP10/HCST in U937 cell line by Flow Cytometry. Human U937 histiocytic lymphoma cell line was stained with Mouse Anti-Human DAP10/HCST Monoclonal Antibody (Catalog # MAB9786, filled histogram) or Mouse IgG2A isotype control antibody (Catalog # MAB003, open histogram) followed by PE-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DAP10/HCST

This gene encodes a transmembrane signaling adaptor that contains a YxxM motif in its cytoplasmic domain. The encoded protein may form part of the immune recognition receptor complex with the C-type lectin-like receptor NKG2D. As part of this receptor complex, this protein may activate phosphatidylinositol 3-kinase dependent signaling pathways through its intracytoplasmic YxxM motif. This receptor complex may have a role in cell survival and proliferation by activation of NK and T cell responses. Alternative splicing results in two transcript variants encoding different isoforms.

Entrez Gene IDs

10870 (Human); 23900 (Mouse); 474146 (Rat)

Alternate Names

DAP10; DAP10PIK3AP; DKFZP586C1522; HCST; hematopoietic cell signal transducer; KAP10; KAP10DNAX-activation protein 10; kinase assoc pro of ~10kDa; Kinase Assoc Protein; Membrane protein DAP10; phosphoinositide-3-kinase adaptor protein; PIK3AP; Transmembrane adapter protein KAP10