Human DAPK3/ZIPK Antibody

Catalog #: MAB5290 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB5290
MAB5290-SP

Detection of Human DAPK3/ZIPK by Western Blot.

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All DAPK3/ZIPK Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human DAPK3/ZIPK Antibody Summary

Species Reactivity

Human

Specificity

Detects human DAPK3/ZIPK in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) DAPK1, rhDAPK2, rhDRAK1, or rhDRAK2 is observed.

Source

Monoclonal Mouse IgG_2B Clone # 729830

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human DAPK3/ZIPK
Arg347-Arg454
Accession # O43293

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

0.5 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human DAPK3/ZIPK by Western Blot. Western blot shows lysates of A431 human epithelial carcinoma cell line, HeLa human cervical epithelial carcinoma cell line, and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human DAPK3/ZIPK Monoclonal Antibody (Catalog # MAB5290) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for DAPK3/ZIPK at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DAPK3/ZIPK

DAPK3 (Death-associated protein kinase 3; also ZIP kinase and DLK) is a 52 kDa member of the CAMK Ser/Thr kinase family, protein kinase superfamily of enzymes. In general, mouse DAPK3 is nuclear while human DAPK3 is cytosolic. DAPK3 is found in smooth muscle where it phosphorylates the regulatory light chain of myosin and stabilizes thick filament formation. Human DAPK3 is 454 amino acids (aa) in length. It contains one protein kinase domain (aa 134-275) and a C‑terminal Leu-zipper motif (aa 427-441) that mediates homo- and hetero-dimerization. There is one 35 kDa alternate splice form that shows a 10 aa substitution for aa 313-454. This form has both a nuclear and cytosolic localization. Over aa 347‑454, human DAPK3 is 65% aa identical to mouse DAPK3.

Long Name

Death-associated Protein Kinase 3

Entrez Gene IDs

1613 (Human); 13144 (Mouse); 64391 (Rat)

Alternate Names

DAP kinase 3; DAPK3; DAP-like kinase; death-associated protein kinase 3; Dlk; EC 2.7.11; EC 2.7.11.1; ZIP kinase isoform; ZIP; ZIPK; ZIPKFLJ36473; ZIP-kinase