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Human DARC PE-conjugated Antibody

Catalog #: FAB4139P
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
FAB4139P
Detection of DARC in Human Red Blood Cells by Flow Cytometry.
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Citations (2)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human DARC PE-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human DARC. Stains human DARC transfected cells but not irrelevant transfectants.
Source
Monoclonal Mouse IgG2A Clone # 358307
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
HEK293 human embryonic kidney cell line transfected with human DARC
Met1-Ser336
Accession # Q16570
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of DARC antibody in Human Red Blood Cells antibody by Flow Cytometry. View Larger

Detection of DARC in Human Red Blood Cells by Flow Cytometry. Human red blood cells were stained with Mouse Anti-Human DARC PE-conjugated Monoclonal Antibody (Catalog # FAB4139P, filled histogram) or isotype control antibody (Catalog # IC003P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: DARC

Duffy Antigen Receptor for Chemokines (DARC), also known as Fy glycoprotein and CD234, is a 7TM non-signaling chemokine receptor that is expressed on venular endothelial cells and erythrocytes. DARC binds and mediates the internalization of several inflammatory CC and CXC chemokines. It transports chemokines to the lumenal face of venular endothelium to promote the extravasation of leukocytes to sites of inflammation. DARC polymorphisms are important in blood transfusion compatibility and determine the susceptibility of erythrocytes to Plasmodium vivax infection. Human DARC shares 60% amino acid sequence identity with mouse DARC.

Long Name
Duffy Antigen, Receptor For Chemokines
Entrez Gene IDs
2532 (Human); 13349 (Mouse)
Alternate Names
ACKR1; CCBP1; CD234 antigen; CD234; DARC; Dfy; Duffy Antigen; Duffy antigen/chemokine receptor; Duffy blood group antigen; Duffy blood group, chemokine receptor; Fy Antigen; Fy glycoprotein; FYDuffy blood group; Glycoprotein D; GPD; GPDWBCQ1; GpFy; Plasmodium vivax receptor

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Citations for Human DARC PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Altered activation state of circulating neutrophils in patients with neovascular age-related macular degeneration
    Authors: Marie Krogh Nielsen, Sven Magnus Hector, Kelly Allen, Yousif Subhi, Torben Lykke Sørensen
    Immunity & Ageing
  2. Extensive ex vivo expansion of functional human erythroid precursors established from umbilical cord blood cells by defined factors.
    Authors: Huang, Xiaosong, Shah, Siddhart, Wang, Jing, Ye, Zhaohui, Dowey, Sarah N, Tsang, Kit Man, Mendelsohn, Laurel G, Kato, Gregory, Kickler, Thomas S, Cheng, Linzhao
    Mol Ther, 2013-09-03;22(2):451-63.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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