Home / DC-SIGN/CD209 / Human DC-SIGN/CD209 APC-conjugated Antibody

Human DC-SIGN/CD209 APC-conjugated Antibody

Catalog #: FAB161A
7 Citations Datasheet / COA / SDS
Clone 120507 was used by HLDA to establish CD designation
Catalog # Availability Size / Price Qty
FAB161A-100
FAB161A-025
Detection of DC‑SIGN/CD209 in NIH‑3T3 Mouse Cell Line Transfected with Human DC-SIGN/CD209 by Flow Cytometry.
1 Image
Product Details
Citations (7)
FAQs
Supplemental Products
Reviews
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human DC-SIGN/CD209 APC-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human DC‑SIGN/CD209 on transfected NIH/3T3 cells and on monocyte derived dendritic cells. Does not react with parental mouse cells or irrelevant transfectants, such as human DC-SIGN2.
Source
Monoclonal Mouse IgG2B Clone # 120507
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NIH-3T3 mouse embryonic fibroblast cell line transfected with human DC‑SIGN/CD209
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of DC-SIGN/CD209 antibody in NIH-3T3 Mouse Cell Line Transfected with Human DC-SIGN/CD209 antibody by Flow Cytometry. View Larger

Detection of DC‑SIGN/CD209 in NIH‑3T3 Mouse Cell Line Transfected with Human DC-SIGN/CD209 by Flow Cytometry. NIH-3T3 mouse embryonic fibroblast cell line transfected with human DC-SIGN/CD209 was stained with Mouse Anti-Human DC-SIGN/CD209 APC-conjugated Monoclonal Antibody (Catalog # FAB161A, filled histogram) or isotype control antibody (Catalog # IC0041A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: DC-SIGN/CD209

Human DC-SIGN (Dendritic Cell-Specific ICAM-3 Grabbing Non-integrin) also known as CD209 is a member of the chromosome 19 C-type lectin family that includes DC-SIGN, DC-SIGN-related protein, CD23 and LSECtin (1). DC-SIGN was initially reported to be a 46 kDa, 404 amino acid (aa) type II transmembrane protein that contained a 40 aa cytoplasmic N-terminus, a 21 aa transmembrane segment, and a 343 aa extracellular C-terminus (2). The extracellular region contains a distal, 115 aa Ca++-dependent carbohydrate-binding lectin domain and a membrane-proximal linker segment that is composed of seven 23 aa repeats (2, 3). The lectin domain is believed to preferably bind mannose, either within the context of ICAM-3 (on T cells) or ICAM-2 (on endothelial cells) (2, 4, 5). DC-SIGN expression appears to be limited to dendritic cells (DC) and macrophages (6), and DC interaction with the ICAMs both aids DC cell trafficking and immunological synapse formation (7). Since the original report on DC-SIGN, multiple splice forms have been discovered, generating both membrane-bound and soluble forms (3). There are eight type A isoforms, all of which begin with the same 15 aa of exon 1a. Four contain the transmembrane region of exon II, and four do not (i.e., are soluble). Among these eight type A isoforms, only three retain the entire 343 aa found in the full length form described in reference #2 (the full length form is referred to as Type I mDC-SIGN1A) (3). Five additional isoforms utilize an alternate start site, and these are referred to as type B isoforms. These all show a 35 aa cytoplasmic domain. One also has a transmembrane segment; four do not. Two of the five contain full, unspliced extracellular regions (3). All of this suggests enormous complexity in DC-SIGN biology. DC-SIGN is not well conserved across species. Human and mouse show little overall aa identity. In the lectin domain, however, human DC-SIGN shares 68% aa identity with mouse DC-SIGN (8). Human and rhesus monkey DC-SIGN share 91% aa identity over the entire extracellular region (8). A detailed description of the additional properties of this monoclonal antibody (MAB161) have been published (9, 10).

References
  1. Liu, W. et al. (2004) J. Biol. Chem. 279:18748.
  2. Curtis, B.M. et al. (1992) Proc. Natl. Acad. Sci. USA 89:8356.
  3. Mummidi, S. et al. (2001) J. Biol. Chem. 276:33196.
  4. Su, S.V. et al. (2004) J. Biol. Chem. 279:19122.
  5. Cambi, A. et al. (2005) Cell. Microbiol. 7:481.
  6. Serrano-Gomez, D. et al. (2004) J. Immunol. 173:5635.
  7. Geijtenbeek, T.B.H. and Y. van Kooyk (2003) Curr. Top. Microbiol. Immunol. 276:32.
  8. Baribaud, F. et al. (2001) J. Virol. 75:10281.
  9. Wu, L. et al. (2002) J. Virol. 76:5905.
  10. Baribaud, F. et al. (2002) J. Virol.76:9135.
Long Name
Dendritic Cell-specific ICAM-3-grabbing Non-integrin 1
Entrez Gene IDs
30835 (Human); 170786 (Mouse); 102121984 (Cynomolgus Monkey)
Alternate Names
CD209 antigendendritic cell-specific intracellular adhesion molecules (ICAM)-3 grabbingnon-integrin; CD209 molecule; CD209; CDSIGNHIV gpl20-binding protein; CLEC4L; CLEC4LC-type lectin domain family 4 member L; DCSIGN; DC-SIGN; DC-SIGN1; DC-SIGN1C-type lectin domain family 4, member L; DC-SIGNMGC129965; Dendritic cell-specific ICAM-3-grabbing non-integrin 1

Product Datasheets

You must select a language.

x
Product Datasheet
COA
SDS

Citations for Human DC-SIGN/CD209 APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
Filter your results:

Filter by:

  1. The Vi Capsular Polysaccharide of Salmonella Typhi Promotes Macrophage Phagocytosis by Binding the Human C-Type Lectin DC-SIGN
    Authors: LF Zhang, B Lepenies, S Nakamae, BM Young, RL Santos, M Raffatellu, BA Cobb, H Hiyoshi, AJ Bäumler
    MBio, 2022-10-26;0(0):e0273322.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Characterization of the Filovirus-Resistant Cell Line SH-SY5Y Reveals Redundant Role of Cell Surface Entry Factors
    Authors: FJ Zapatero-B, E Dietzel, O Dolnik, K Döhner, R Costa, B Hertel, B Veselkova, J Kirui, A Klintworth, MP Manns, S Pöhlmann, T Pietschman, T Krey, S Ciesek, G Gerold, B Sodeik, S Becker, T von Hahn
    Viruses, 2019-03-19;11(3):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Differentiation of Langerhans Cells from Monocytes and Their Specific Function in Inducing IL-22-Specific Th Cells
    Authors: Y Otsuka, E Watanabe, E Shinya, S Okura, H Saeki, TBH Geijtenbee, H Takahashi
    J. Immunol., 2018-10-15;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Inefficient HIV-1 trans infection of CD4+ T cells by macrophages from HIV-1 nonprogressors is associated with altered membrane cholesterol and DC-SIGN
    Authors: DC DeLucia, CR Rinaldo, G Rappocciol
    J. Virol., 2018-06-13;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Dendritic cells from the human female reproductive tract rapidly capture and respond to HIV
    Mucosal Immunol, 2016-08-31;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Transcutaneous immunization as preventative and therapeutic regimens to protect against experimental otitis media due to nontypeable Haemophilus influenzae.
    Authors: Novotny LA, Clements JD, Bakaletz LO
    Mucosal Immunol, 2011-02-16;4(4):456-67.
    Species: Chinchilla
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. A Prominent Role for DC-SIGN+ Dendritic Cells in Initiation and Dissemination of Measles Virus Infection in Non-Human Primates
    Authors: Annelies W. Mesman, Rory D. de Vries, Stephen McQuaid, W. Paul Duprex, Rik L. de Swart, Teunis B. H. Geijtenbeek
    PLoS ONE

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for Human DC-SIGN/CD209 APC-conjugated Antibody

There are currently no reviews for this product. Be the first to review Human DC-SIGN/CD209 APC-conjugated Antibody and earn rewards!

Have you used Human DC-SIGN/CD209 APC-conjugated Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review