Human DC-SIGN+DC-SIGNR Antibody Summary
Extracellular domain
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: DC-SIGN+DC-SIGNR
DC-SIGN (Dendritic Cell- Specific ICAM-3 Grabbing Non-Integrin) has been shown to play an important role in regulating dendritic cell (DC) and T cell interactions, including antigen presentation to T cells and enhancement of transinfection of CD4+ T cells by HIV-1 (1, 2). Efforts to identify additional type II membrane proteins resulted in the isolation of a molecule related in sequence to DC-SIGN known as DC-SIGNR (DC-SIGN Related) (3, 4). DC-SIGNR shares 73 - 80% amino acid homology with DC-SIGN and is located on human chromosome 19p13.3. Its structure is similar to DC-SIGN and therefore binds mannose residues in a calcium dependent fashion, including ICAM-3 and HIV-1 gp120 (5). DC-SIGNR, also known as L-SIGN (Liver/Lymph node-Specific ICAM-3-Grabbing Non-integrin) and DC‑SIGNR, is polymorphic since allelic variations of the exon 4 encoded sequence have been isolated (5). This is further supported by a study demonstrating the ability to isolate a large repertoire of DC-SIGNR transcripts largely the result of alternative splicing of the 7 coding exons (6). L-SIGN/DC-SIGNR is primarily transcribed in the liver and lymph nodes but not in monocyte derived DC (5). Expression of L-SIGN/DC-SIGNR is restricted to endothelial cells derived from liver sinusoids, lymph nodes sinuses and capillaries (7) although variable expression in placenta and some monocytic cell lines has also been reported, including both membrane and soluble isoforms of the protein (6). Expression of DC-SIGN is induced during the in-vitro generation of DC from either monocytes or bone marrow progenitors, with maximal surface expression at day 7 of culture (1). Immature DC in the skin and mature DC in the tonsil have been demonstrated to express DC‑SIGN (8). Analysis of various tissues and cell lines suggests that DC-SIGN expression is restricted to DC (1) although a more recent report finds evidence of expression in placenta, resting monocytes and monocytic cell lines (6). This discrepancy may be partially related to the multiple isoforms of DC-SIGN transcripts, including both membrane and soluble forms, as well as exon splice variants reported in the latter study (6). A detailed description of the additional properties of this monoclonal antibody may be found in references 9 and 10.
- Geijtenbeek, T.B.H. et al. (2000) Cell 100:575.
- Geijtenbeek, T.B.H. et al. (2000) Cell 100:587.
- Yokoyama-Kobayashi, M.T. et al. (1999) Gene 228:161.
- Soilleux, E.J. et al. (2000) J. Immunol. 165:2937.
- Bashirova, A.A. et al. (2001) J. Exp. Med. 193:671.
- Mummidi, S. et al. (2001) J. Biol. Chem., 2001 May 3 [epub ahead of print].
- Pohlman, S. et al. (2001) Proc. Natl. Acad. Sci. USA 98:2670.
- Geijtenbeek, T.B.H. et al. (2000) Nature Immunol. 1:353.
- Wu, L. et al. (2002) J. Virol. 76:5905.
- Baribaud, F. et al. (2002) J. Virol. 76:9135.
Product Datasheets
Citations for Human DC-SIGN+DC-SIGNR Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 6
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Overexpression of dendritic cell-specific intercellular adhesion molecule-3-grabbing nonintegrin-related protein in cervical cancer and correlation with squamous cell carcinoma antigen
Authors: Xiangdong Wang, Yangmei Jiang, Menglang Yuan, Chunlin Chen, Keyong Wang, Qianshi Zhang et al.
Oncology Letters
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DC/L-SIGN recognition of spike glycoprotein promotes SARS-CoV-2 trans-infection and can be inhibited by a glycomimetic antagonist
Authors: Michel Thépaut, Joanna Luczkowiak, Corinne Vivès, Nuria Labiod, Isabelle Bally, Fátima Lasala et al.
PLOS Pathogens
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N-glycosylation of viral glycoprotein is a novel determinant for the tropism and virulence of highly pathogenic tick-borne bunyaviruses
Authors: Shimojima, M;Sugimoto, S;Taniguchi, S;Maeki, T;Yoshikawa, T;Kurosu, T;Tajima, S;Lim, CK;Ebihara, H;
PLoS pathogens
Species: Human, Primate - Cercopithecus aethiops (African Green Monkey)
Sample Types: Whole Cells
Applications: Flow Cytometry -
Efficient functional screening of a cellular cDNA library to identify severe fever with thrombocytopenia syndrome virus entry factors
Authors: M Shimojima, S Sugimoto, S Taniguchi, T Yoshikawa, T Kurosu, M Saijo
Sci Rep, 2020-04-07;10(1):5996.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
CCR5-, DC-SIGN-dependent endocytosis and delayed reverse transcription after human immunodeficiency virus type 1 infection in human astrocytes.
Authors: Deiva K, Khiati A, Hery C, Salim H, Leclerc P, Horellou P, Tardieu M
AIDS Res. Hum. Retroviruses, 2006-11-01;22(11):1152-61.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry, ICC, Neutralization -
L-SIGN (CD209L) isoforms differently mediate trans-infection of hepatoma cells by hepatitis C virus pseudoparticles.
Authors: Falkowska E, Durso R, Gardner J, Cormier E, Arrigale R, Ogawa R, Donovan G, Maddon P, Olson W, Dragic T
J Gen Virol, 2006-09-01;87(0):2571-6.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
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